Eker Bilge, Yilmaz Mahmut Deniz, Schlautmann Stefan, Gardeniers Johannes G E, Huskens Jurriaan
Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, 7500 AE, Enschede, The Netherlands; E-Mails:
Int J Mol Sci. 2011;12(11):7335-51. doi: 10.3390/ijms12117335. Epub 2011 Oct 26.
A supramolecular platform based on self-assembled monolayers (SAMs) has been implemented in a microfluidic device. The system has been applied for the sensing of two different analyte types: biologically relevant phosphate anions and aromatic carboxylic acids, which are important for anthrax detection. A Eu(III)-EDTA complex was bound to β-cyclodextrin monolayers via orthogonal supramolecular host-guest interactions. The self-assembly of the Eu(III)-EDTA conjugate and naphthalene β-diketone as an antenna resulted in the formation of a highly luminescent lanthanide complex on the microchannel surface. Detection of different phosphate anions and aromatic carboxylic acids was demonstrated by monitoring the decrease in red emission following displacement of the antenna by the analyte. Among these analytes, adenosine triphosphate (ATP) and pyrophosphate, as well as dipicolinic acid (DPA) which is a biomarker for anthrax, showed a strong response. Parallel fabrication of five sensing SAMs in a single multichannel chip was performed, as a first demonstration of phosphate and carboxylic acid screening in a multiplexed format that allows a general detection platform for both analyte systems in a single test run with μM and nM detection sensitivity for ATP and DPA, respectively.
一种基于自组装单分子层（SAMs）的超分子平台已应用于微流控装置中。该系统已用于检测两种不同类型的分析物：对炭疽检测很重要的生物相关磷酸根阴离子和芳香族羧酸。通过正交超分子主客体相互作用，将铕（III）-乙二胺四乙酸（EDTA）络合物与β-环糊精单分子层结合。铕（III）-EDTA共轭物与作为天线的萘β-二酮自组装，在微通道表面形成了高发光性的镧系元素络合物。通过监测分析物取代天线后红色发射的减少，证明了对不同磷酸根阴离子和芳香族羧酸的检测。在这些分析物中，三磷酸腺苷（ATP）、焦磷酸以及作为炭疽生物标志物的吡啶二羧酸（DPA）显示出强烈的响应。在单个多通道芯片中并行制备了五个传感SAMs，这首次证明了以多重形式进行磷酸根和羧酸的筛选，该筛选允许在一次测试运行中为两种分析物系统构建通用检测平台，对ATP和DPA的检测灵敏度分别为微摩尔和纳摩尔级别。
