Paillot R, Prowse L
Animal Health Trust, Centre for Preventive Medicine, Lanwades Park, Newmarket, Suffolk CB8 7UU, UK.
Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):516-21. doi: 10.1016/j.vetimm.2011.11.019. Epub 2011 Nov 26.
The humoral immune response induced by ISCOM-matrix (Immuno Stimulating COMplex-Matrix)-adjuvanted equine influenza virus (EIV) vaccine is well documented in horses. ISCOM-matrix adjuvanted vaccines against human influenza are strong inducers of cell-mediated immunity (CMI), including T cell proliferation and virus-specific cytotoxic T cell. In the horse, the CMI response to equine influenza vaccination is less well characterised. An ISCOM-based vaccine has been shown to induce interferon gamma (IFN-γ) synthesis, a CMI marker, in the horse, but this has not been shown for the ISCOM-matrix vaccine, which is a different formulation. The objective of this study was to measure EIV-specific IFN-γ synthesis after vaccination with an ISCOM-matrix-adjuvanted EIV vaccine. Equilis Prequenza is a commercialised inactivated EIV vaccine containing purified haemagglutinin (HA) and neuraminidase (NA) subunits adjuvanted with ISCOM-matrix. Six influenza-naïve Welsh mountain ponies were vaccinated twice with Equilis Prequenza at an interval of four weeks. Six control ponies received a placebo of physiological water. EIV-specific IFN-γ synthesis by peripheral blood lymphocytes and the antibody response to a panel of representative EIV isolates were measured prior to and after both injections. Immunisation with the ISCOM-matrix-based EIV vaccine stimulated significant EIV-specific IFN-γ synthesis and EIV-specific single radial haemolysis (SRH) antibody. In conclusion, EIV vaccine adjuvanted with ISCOM-matrix stimulates both antibody and a cellular immune response in the horse.
免疫刺激复合物基质（ISCOM-matrix）佐剂的马流感病毒（EIV）疫苗诱导的体液免疫反应在马匹中已有充分记录。针对人类流感的ISCOM-matrix佐剂疫苗是细胞介导免疫（CMI）的强力诱导剂，包括T细胞增殖和病毒特异性细胞毒性T细胞。在马匹中，对马流感疫苗接种的CMI反应特征尚不明确。一种基于ISCOM的疫苗已被证明能在马体内诱导干扰素γ（IFN-γ）合成，这是一种CMI标志物，但ISCOM-matrix疫苗（一种不同的配方）尚未有此报道。本研究的目的是测量接种ISCOM-matrix佐剂的EIV疫苗后EIV特异性IFN-γ的合成。Equilis Prequenza是一种商业化的灭活EIV疫苗，含有纯化的血凝素（HA）和神经氨酸酶（NA）亚单位，并佐以ISCOM-matrix。6匹未感染流感的威尔士山地小马用Equilis Prequenza进行了两次接种，间隔4周。6匹对照小马接受了生理盐水安慰剂。在两次注射前后分别测量外周血淋巴细胞的EIV特异性IFN-γ合成以及对一组代表性EIV分离株的抗体反应。基于ISCOM-matrix的EIV疫苗免疫刺激了显著的EIV特异性IFN-γ合成和EIV特异性单向辐射溶血（SRH）抗体。总之，ISCOM-matrix佐剂的EIV疫苗在马体内刺激了抗体和细胞免疫反应。
