利用 Illumina 测序技术对重组 CHO 细胞系中保守 microRNA 的表达进行分析。
Profiling conserved microRNA expression in recombinant CHO cell lines using Illumina sequencing.
机构信息
Department of Chemical Engineering, University of Delaware, Newark, Delaware, USA.
出版信息
Biotechnol Bioeng. 2012 Jun;109(6):1371-5. doi: 10.1002/bit.24415. Epub 2012 Jan 23.
Abstract
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate multiple aspects of cell physiology. The differential expression of conserved miRNAs in two Chinese hamster ovary (CHO) cell lines producing recombinant proteins was examined relative to the CHO-K1 cell line. A total of 190 conserved CHO miRNAs were identified through homology with known human and rodent miRNAs. More than 80% of these miRNAs showed differential expression in recombinant CHO cell lines. The small RNA sequencing data were analyzed in context of the CHO-K1 genome to examine miRNA organization and develop sequence-specific miRNA resources for CHO cells. The identification and characterization of CHO miRNAs will facilitate the use of miRNA tools in cell line engineering efforts to improve product yield and quality.
摘要
微小 RNA（miRNAs）是调节细胞生理多个方面的小型非编码 RNA。相对于 CHO-K1 细胞系，对产生重组蛋白的两个中国仓鼠卵巢（CHO）细胞系中保守 miRNAs 的差异表达进行了研究。通过与已知的人和啮齿动物 miRNAs 的同源性，鉴定出了总共 190 个保守的 CHO miRNAs。这些 miRNAs 中有超过 80%在重组 CHO 细胞系中表现出差异表达。根据 CHO-K1 基因组对小 RNA 测序数据进行了分析，以研究 miRNA 组织并为 CHO 细胞开发序列特异性 miRNA 资源。CHO miRNAs 的鉴定和表征将有助于在细胞系工程中使用 miRNA 工具来提高产品产量和质量。
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本文引用的文献
1
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.
Nat Biotechnol. 2011 Jul 31;29(8):735-41. doi: 10.1038/nbt.1932.
2
MiR-1 is a tumor suppressor in thyroid carcinogenesis targeting CCND2, CXCR4, and SDF-1alpha.
J Clin Endocrinol Metab. 2011 Sep;96(9):E1388-98. doi: 10.1210/jc.2011-0345. Epub 2011 Jul 13.
3
Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures.
Biotechnol J. 2012 Apr;7(4):500-15. doi: 10.1002/biot.201100143. Epub 2011 Aug 5.
4
miR-30b/30d regulation of GalNAc transferases enhances invasion and immunosuppression during metastasis.
Cancer Cell. 2011 Jul 12;20(1):104-18. doi: 10.1016/j.ccr.2011.05.027.
5
Profiling highly conserved microrna expression in recombinant IgG-producing and parental Chinese hamster ovary cells.
Biotechnol Prog. 2011 Jul;27(4):1163-71. doi: 10.1002/btpr.556. Epub 2011 Jun 20.
6
Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering.
J Biotechnol. 2011 Apr 20;153(1-2):62-75. doi: 10.1016/j.jbiotec.2011.02.011. Epub 2011 Mar 30.
7
Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology.
BMC Genomics. 2011 Jan 26;12:67. doi: 10.1186/1471-2164-12-67.
8
Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7.
J Biotechnol. 2011 Jan 20;151(2):204-11. doi: 10.1016/j.jbiotec.2010.12.005. Epub 2010 Dec 15.
9
MicroRNA-210 regulates cancer cell proliferation through targeting fibroblast growth factor receptor-like 1 (FGFRL1).
J Biol Chem. 2011 Jan 7;286(1):420-8. doi: 10.1074/jbc.M110.170852. Epub 2010 Nov 2.
10
miRBase: integrating microRNA annotation and deep-sequencing data.
Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7. doi: 10.1093/nar/gkq1027. Epub 2010 Oct 30.
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