Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
J Mater Sci Mater Med. 2012 Mar;23(3):763-70. doi: 10.1007/s10856-011-4528-8. Epub 2011 Dec 22.
In this study, biodegradation and biocompatibility of novel poly(ε-caparolactone)/nano fluoridated hydroxyapatite (PCL-FHA) scaffolds were investigated. The FHA nanopowders were prepared via mechanical alloying method and had a chemical composition of Ca(10)(PO(4))(6)OH(2-x )F(x) (where x values were selected equal to 0.5 and 2.0). In order to fabricate PCL-FHA scaffolds, 10, 20, 30 and 40 wt% of the FHA were added to the PCL. The PCL-FHA scaffolds were produced by the solvent casting/particulate leaching using sodium chloride particles (with diameters of 300-500 μm) as the porogen. The phase structure, microstructure and morphology of the scaffolds were evaluated using X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy techniques. Porosity of the scaffolds was measured using the Archimedes' Principle. In vitro degradation of PCL-FHA scaffolds was studied by incubating the samples in phosphate buffered saline at 37°C and pH 7.4 for 30 days. Moreover, biocompatibility was evaluated by MTT assay after seeding and culture of osteoblast-like cells on the scaffolds. Results showed that the osteoblast-like cells attached to and proliferated on PCL-FHA and increasing the porosity of the scaffolds increased the cell viability. Also, degradation rate of scaffolds were increased with increasing the fluorine content in scaffolds composition.
本研究考察了新型聚（ε-己内酯）/纳米氟化羟基磷灰石（PCL-FHA）支架的生物降解性和生物相容性。FHA 纳米粉末通过机械合金化法制备，其化学组成为 Ca(10)(PO(4))(6)OH(2-x )F(x)（其中 x 值选择为 0.5 和 2.0）。为了制备 PCL-FHA 支架，将 10、20、30 和 40wt%的 FHA 添加到 PCL 中。PCL-FHA 支架通过溶剂浇铸/颗粒沥滤法使用直径为 300-500μm 的氯化钠颗粒作为成孔剂来制备。采用 X 射线衍射、傅里叶变换红外光谱和扫描电子显微镜技术评估支架的相结构、微观结构和形貌。通过阿基米德原理测量支架的孔隙率。将 PCL-FHA 支架在 37°C 和 pH7.4 的磷酸盐缓冲盐溶液中孵育 30 天，研究其体外降解情况。此外，通过在支架上接种和培养成骨样细胞后进行 MTT 测定来评估生物相容性。结果表明，成骨样细胞附着在 PCL-FHA 上并增殖，增加支架的孔隙率可以提高细胞活力。此外，随着支架组成中氟含量的增加，支架的降解速率也增加。
