Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
J Microbiol. 2011 Dec;49(6):1027-32. doi: 10.1007/s12275-011-1049-6. Epub 2011 Dec 28.
A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6(T), was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6(T) is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C(15:0) and summed feature 3 (containing C(16:1) ω7c/iso-C(15:0) 2OH, and/or iso-C(15:0) 2OH/C(16:1) ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6(T) and B. cereus group was found to be in a range of 22.8-42.3%, and thus BL4-6(T) represents a unique species. On the basis of these studies, strain BL4-6(T) (=KCTC 13319(T) =JCM 15802(T)) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.
一株黄海潮滩沉积物中分离得到的革兰氏阳性、产芽孢的新 Bacillus 种，菌株 BL4-6(T)。菌株 BL4-6(T)为直杆状，周身鞭毛运动。细胞壁含有 meso-二氨基庚二酸，主要呼吸醌为menaquinone-7。主要脂肪酸为 iso-C(15:0)和 summed feature 3（含有 C(16:1) ω7c/iso-C(15:0) 2OH，和/或 iso-C(15:0) 2OH/C(16:1) ω7c）。细胞过氧化氢酶阳性，氧化酶阴性。基因组 DNA 的 G+C 含量为 38.0 mol%。基于 16S rRNA 基因序列的比较分析，该分离株属于 Bacillus 属，与 Bacillus cereus 群形成一个分支，与 Bacillus mycoides（98.5%）、Bacillus cereus（98.5%）、Bacillus anthracis（98.4%）、Bacillus thuringiensis（98.4%）、Bacillus weihenstephanensis（98.1%）和 Bacillus pseudomycoides（97.5%）最为相近。该菌株 gyrA 基因序列与该群成员的相似性小于 85%，rpoB 基因序列的相似性低于 95%。菌株 BL4-6(T)与 Bacillus cereus 群之间的 DNA-DNA 同源性在 22.8-42.3%之间，因此 BL4-6(T)代表一个独特的种。基于这些研究，菌株 BL4-6(T)（=KCTC 13319(T)=JCM 15802(T)）被提议代表一种新型 Bacillus 属物种的模式菌株，Bacillus manliponensis sp. nov.
