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根管内粪肠球菌成熟生物膜的形成及其对某些根管内抗菌药物的敏感性:一项体外研究

Development of an intracanal mature Enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study.

作者信息

Saber Shehab El-Din Mohamed, El-Hady Soha A

机构信息

Department of Endodontic, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

出版信息

Eur J Dent. 2012 Jan;6(1):43-50.

Abstract

OBJECTIVES

To develop a mature biofilm of Enterococcus faecalis inside the root canal system and to test its susceptibility to some antimicrobial medications in vitro.

METHODS

Single rooted premolars were mechanically enlarged, sterilized, and then infected with a clinical isolate of E. faecalis. Biofilm formation and maturation was monitored using SEM. Biofilm bacteria were exposed to Amoxicillin+clavulanate, Ciprofloxacin, Clindamycin, Doxycycline, and calcium hydroxide as intracanal medications for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated.

RESULTS

SEM examination confirmed the formation of a mature biofilm at the end of the incubation period. All the chemotherapeutic agents used were significantly better than Calcium hydroxide in elimination of biofilm bacteria. The antimicrobial effect of Amoxicillin + clavulanate, Ciprofloxacin and Clindamycin was significantly better than Doxycycline (P=.05). However the difference in the antimicrobial effectiveness among them was statistically non-significant (P=.05).

CONCLUSIONS

The method used for bacterial biofilm development and maturation is reliable and can be used to assess the anti bacterial potential of endodontic materials. Also, the local application of antibacterial agents can be beneficial in resistant cases of apical periodontitis but only after careful culture and sensitivity testing to choose the appropriate agent for the existing flora.

摘要

目的

在根管系统内培养成熟的粪肠球菌生物膜,并在体外测试其对某些抗菌药物的敏感性。

方法

对单根前磨牙进行机械扩锉、消毒,然后用粪肠球菌临床分离株进行感染。使用扫描电子显微镜监测生物膜的形成和成熟情况。将生物膜细菌作为根管内用药,分别暴露于阿莫西林+克拉维酸、环丙沙星、克林霉素、多西环素和氢氧化钙中1周。最后收集细菌样本并计数菌落形成单位。

结果

扫描电子显微镜检查证实培养期结束时形成了成熟的生物膜。所有使用的化疗药物在清除生物膜细菌方面均明显优于氢氧化钙。阿莫西林+克拉维酸、环丙沙星和克林霉素的抗菌效果明显优于多西环素(P = 0.05)。然而,它们之间抗菌效果的差异在统计学上无显著意义(P = 0.05)。

结论

用于细菌生物膜培养和成熟的方法可靠,可用于评估牙髓材料的抗菌潜力。此外,局部应用抗菌剂在根尖周炎耐药病例中可能有益,但前提是要经过仔细的培养和药敏试验,以选择适合现有菌群的药物。

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