College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.
Pharm Biol. 2012 Feb;50(2):225-31. doi: 10.3109/13880209.2011.596207.
The rhizome of Wikstroemia indica (L.) C. A. Mey (Thymelaeaceae) is widespread in China which has been widely used in China as folk medicine for the treatment of syphilis, arthritis, whooping cough, and cancer. Due to its multiactivities, its extract has an attractive potential as a promising natural agent in the pharmaceutical industries.
Aims of this study were to optimize the extraction process of the flavonoids from W. indica, and evaluate its multiple activities.
An orthogonal test design was employed to optimize the extraction procedure of flavonoids from W. indica. And multichromatography and spectroscopy were used to study the chemical compounds of W. indica, while several bioactivity assays were used to evaluate the antibacterial, anti-inflammatory, and antitumor activities of W. indica.
Optimal extraction conditions were determined: ethanol concentration was 60%; extraction time was 60 min; liquid-solid ratio was 16:1 and the power of ultrasonic instrument was 160 W. Four compounds: daphnoretin, chrysophanol, myricitrime and rutin were purified from W. indica, and chrysophanol was identified from this plant for the first time. The extract of W. indica displayed significant antimicrobial and anti-inflammatory activities. Daphnoretin showed a significant inhibition effect on CNE cells and HeLa cells lines at the concentrations ranging from 15.6 to 125 μg/mL, the tendency of antitumor effect was displayed in a concentration-dependent manner.
Extracts of W. indica could potentially be used as a promising natural agent in the pharmaceutical industries.
野牡丹（Wikstroemia indica）的根茎在中国广泛分布，被广泛用作民间药物治疗梅毒、关节炎、百日咳和癌症。由于其多效性，其提取物作为一种有吸引力的潜在天然药物在制药行业中具有广阔的应用前景。
本研究旨在优化野牡丹中黄酮类化合物的提取工艺，并评价其多种活性。
采用正交试验设计优化野牡丹中黄酮类化合物的提取工艺。采用多种层析和光谱法研究野牡丹的化学成分，同时采用多种生物活性测定方法评价野牡丹的抗菌、抗炎和抗肿瘤活性。
确定了最佳提取条件：乙醇浓度为 60%；提取时间为 60 分钟；液固比为 16:1，超声仪器功率为 160 W。从野牡丹中分离得到 4 种化合物：瑞香素、大黄素、杨梅素和芦丁，并首次从该植物中鉴定出大黄素。野牡丹提取物显示出显著的抗菌和抗炎活性。瑞香素在 15.6 至 125 μg/mL 的浓度范围内对 CNE 细胞和 HeLa 细胞系表现出显著的抑制作用，其抗肿瘤作用呈浓度依赖性。
野牡丹提取物有望成为制药行业有前途的天然药物。
