Institute of Medical Science, University of Toronto, Toronto, Canada.
Int J Med Sci. 2012;9(2):157-62. doi: 10.7150/ijms.3880. Epub 2012 Jan 13.
In vitro, nitric oxide (NO) has been shown to have antimicrobial activity against a wide range of viruses, including influenza A virus. Therefore, we hypothesized that inhaled nitric oxide (iNO) would increase survival in vivo by reducing the viral load in C57Bl/6 mice infected with a lethal dose of influenza A/WSN/33 (H1N1; WSN/33) virus. NO was delivered to influenza-infected mice either continuously or intermittently at 80 or 160 ppm, respectively, using both prophylactic and post-infection treatment strategies. Murine survival and weight loss were assessed, and lung viral load was quantified via plaque assay. Here, we report that iNO administered prophylactically or post-influenza infection failed to improve survival of infected mice. No difference in lung viral load was observed between experimental groups. Although NO has antiviral activity against influenza A virus in vitro, iNO therapy provided no apparent benefit when used for treatment of influenza A virus infection in vivo.
在体外，一氧化氮 (NO) 已被证明对多种病毒具有抗菌活性，包括甲型流感病毒。因此，我们假设吸入一氧化氮 (iNO) 通过降低感染致死剂量甲型流感 A/WSN/33（H1N1；WSN/33）病毒的 C57Bl/6 小鼠中的病毒载量，会增加体内存活率。使用持续或间歇性两种方式，分别以 80 或 160ppm 的浓度将 NO 输送给感染流感的小鼠，采用预防和感染后治疗两种策略。通过噬菌斑分析来评估小鼠的存活率和体重减轻情况，并定量检测肺部病毒载量。在这里，我们报告称，预防性或感染后给予 iNO 均未能改善感染小鼠的存活率。实验组之间的肺部病毒载量没有差异。尽管 NO 对甲型流感病毒在体外具有抗病毒活性，但在用于治疗体内甲型流感病毒感染时，iNO 治疗并没有明显的益处。
