转录因子 STOX1A 通过与 CCNB1 启动子结合促进有丝分裂进入。
Transcription factor STOX1A promotes mitotic entry by binding to the CCNB1 promotor.
机构信息
Department of Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands.
出版信息
PLoS One. 2012;7(1):e29769. doi: 10.1371/journal.pone.0029769. Epub 2012 Jan 13.
BACKGROUND
In this study we investigated the involvement of the transcription factor STOX1A in the regulation of the cell cycle.
METHODOLOGY/PRINCIPAL FINDINGS: We found that several major cell cycle regulatory genes were differentially expressed upon STOX1A stimulation and knockdown in the neuroblastoma cell line SH-SY5Y. This includes STOX1A dependent differential regulation of cyclin B1 expression, a cyclin which is known to regulate mitotic entry during the cell cycle. The differential regulation of cyclin B1 expression by STOX1A is direct as shown with chromatin immunoprecipitation. Results furthermore suggest that mitotic entry is enhanced through the direct upregulation of cyclin B1 expression effectuated by STOX1A.
CONCLUSIONS
In conclusion we hereby show that STOX1A is directly involved in the regulation of the cell cycle.
背景
在这项研究中，我们调查了转录因子 STOX1A 在细胞周期调控中的作用。
方法/主要发现：我们发现，在神经母细胞瘤细胞系 SH-SY5Y 中，STOX1A 的刺激和敲低会导致几个主要的细胞周期调节基因的差异表达。这包括 STOX1A 对细胞周期中调节有丝分裂进入的细胞周期蛋白 B1 表达的差异调节。染色质免疫沉淀显示，STOX1A 对细胞周期蛋白 B1 表达的差异调节是直接的。结果还表明，通过 STOX1A 直接上调细胞周期蛋白 B1 的表达，促进有丝分裂的进入。
结论
总之，我们证明了 STOX1A 直接参与细胞周期的调控。
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