Department of Biological Chemistry and Molecular Modeling, Institute of Advanced Chemistry of Catalonia (IQAC - CSIC), Barcelona, Spain.
Biophys J. 2011 Dec 7;101(11):2782-9. doi: 10.1016/j.bpj.2011.09.033.
The dynamical basis underlying the increased thermal stability of thermophilic proteins remains uncertain. Here, we challenge the new paradigm established by neutron scattering experiments in solution, in which the adaptation of thermophilic proteins to high temperatures lies in the lower sensitivity of their flexibility to temperature changes. By means of a combination of molecular dynamics and Brownian dynamics simulations, we report a reinterpretation of those experiments and show evidence that under crowding conditions, such as in vivo, thermophilic and homolog mesophilic proteins have diffusional properties with different thermal behavior.
热稳定性增加的热稳定性的动力学基础仍然不确定。在这里，我们挑战了在溶液中进行的中子散射实验所建立的新范例，其中，嗜热蛋白对高温的适应在于其柔韧性对温度变化的敏感性较低。通过分子动力学和布朗动力学模拟的结合，我们重新解释了这些实验，并证明了在拥挤的条件下，如在体内，嗜热和同源嗜中温蛋白具有不同热行为的扩散性质。
