用专业强度氟化钠局部糊剂处理的脱矿牙本质的扫描电子显微镜评估

SEM evaluation of demineralized dentin treated with professional-strength NaF topical pastes.

作者信息

Karlinsey Robert L, Mackey Allen C, Schwandt Craig S, Walker Trenton J

机构信息

Indiana Nanotech, 351 West 10th Street, Suite 309, Indianapolis, IN 46202, USA.

出版信息

Am J Dent. 2011 Dec;24(6):357-62.

PMID:22263333

Abstract

PURPOSE

To qualitatively explore the effects of prescription-strength topical NaF pastes on patent tubules in a pH cycling model comprising remineralization and demineralization phases.

METHODS

3 mm diameter dentin cores were extracted from bovine teeth, mounted in acrylic rods, ground and polished, and initially demineralized in 10 mL unstirred 50% citric acid (pH = 1.2) for 2 minutes to remove the smear layer and expose the dentin tubules. 72 acceptable specimens were then divided into six groups (12 per group) and subjected to a two-phase pH cycling study. The six pastes evaluated were as follows: fluoride-free Silly Strawberry, Clinpro 5000 and Clinpro Tooth Crème, MI Paste Plus, Sensodyne NUPRO 5000 and Topex Renew. First, the specimens were subjected to a remineralization regimen (Phase A), with each day comprising inverted immersion of specimens in three paste treatments (10 g paste: 20 mL distilled water) performed for 1 minute apiece with the immersion in artificial saliva (pH = 7.0) for 2 hours in between each treatment as well as overnight. After 7 days, the specimens were imaged using field emission scanning electron microscopy (FESEM). The remaining six specimens from each group were then cycled through a demineralization regimen (Phase B), with each day comprising inverted immersion of specimens in three rounds of a 1-minute treatment (10 g paste: 20 ml distilled water) followed immediately by a 3-minute static acid challenge (0.3% citric acid, pH = 3.8) with 2 hours in artificial saliva in between each round as well as overnight. After 3 days, the specimens were again imaged using FESEM.

RESULTS

After the Phase A regimen (i.e. remineralization phase), treatment with Silly Strawberry (fluoride-free), Sensodyne NUPRO 5000 and Topex Renew yielded qualitatively similar tubule pore appearance, whereby deposits appeared to have formed around the tubule openings. Clinpro 5000, MI Paste Plus and Clinpro Tooth Crème appeared to occlude dentin tubules as shown by the relatively smaller diameter tubule openings. Relative comparisons among these pastes revealed Clinpro 5000 appeared especially effective in narrowing and incorporating mineral within the tubule, while the MI Paste Plus and Clinpro Tooth Crème appear to produce comparable tubule occlusion. After the Phase B regimen (i.e. resistance to demineralization), qualitative comparisons revealed the fluoride-free, MI Paste Plus, NUPRO and Renew pastes did not appear to occlude dentin tubules as well as Clinpro 5000 and Clinpro Tooth Crème.

摘要

目的

在包含再矿化和脱矿化阶段的pH循环模型中，定性探究处方强度的局部用氟化钠糊剂对开放牙本质小管的影响。

方法

从牛牙中提取直径3毫米的牙本质核，安装在丙烯酸棒中，进行研磨和抛光，最初在10毫升未搅拌的50%柠檬酸（pH = 1.2）中脱矿2分钟，以去除玷污层并暴露牙本质小管。然后将72个合格标本分为六组（每组12个），进行两阶段pH循环研究。评估的六种糊剂如下：不含氟的Silly Strawberry、Clinpro 5000和Clinpro Tooth Crème、MI Paste Plus、舒适达NUPRO 5000和Topex Renew。首先，将标本进行再矿化方案（A阶段），每天包括将标本倒置浸泡在三种糊剂处理中（10克糊剂：20毫升蒸馏水），每次处理1分钟，每次处理之间在人工唾液（pH = 7.0）中浸泡2小时以及过夜。7天后，使用场发射扫描电子显微镜（FESEM）对标本进行成像。然后，每组剩余的六个标本进行脱矿化方案（B阶段）循环，每天包括将标本倒置浸泡在三轮1分钟的处理中（10克糊剂：20毫升蒸馏水），随后立即进行3分钟的静态酸刺激（0.3%柠檬酸，pH = 3.8），每轮之间在人工唾液中浸泡2小时以及过夜。3天后，再次使用FESEM对标本进行成像。

结果

在A阶段方案（即再矿化阶段）后，用Silly Strawberry（不含氟）、舒适达NUPRO 5000和Topex Renew处理后，小管孔隙外观在质量上相似，即小管开口周围似乎形成了沉积物。Clinpro 5000、MI Paste Plus和Clinpro Tooth Crème似乎封闭了牙本质小管，如直径相对较小的小管开口所示。这些糊剂之间的相对比较显示，Clinpro 5000在使小管变窄并在小管内结合矿物质方面似乎特别有效，而MI Paste Plus和Clinpro Tooth Crème似乎产生了相当的小管封闭效果。在B阶段方案（即抗脱矿化）后，定性比较显示，不含氟的、MI Paste Plus、NUPRO和Renew糊剂在封闭牙本质小管方面似乎不如Clinpro 5000和Clinpro Tooth Crème。