Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Beijing 100850, PR China.
J Pharm Biomed Anal. 2012 Mar 25;62:235-49. doi: 10.1016/j.jpba.2011.12.027. Epub 2012 Jan 2.
Steroidal saponins in Rhizoma Paridis attract scientific attentions for their structural diversity and significant bioactivities. In this work, an ultra performance liquid chromatography coupled with a hybrid quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) was used to rapidly separate and identify steroidal saponins from the extract of the rhizome of Paris polyphylla var. yunnanensis (PPY). The fragment ions from glycosidic and cross-ring cleavages offered a wealth of structural information that is indicative to the aglycones, sugar types and the connecting sequence of sugar units. Based on the exact mass information, the fragmentation characteristics, and the LC retention times of 21 reference steroidal saponin standards, 98 constituents were tentatively identified with their structures proposed, which covered more than 30 types of steroidal aglycones. The 98 constituents consist of 22 pairs of structural isomers, and 40 steroidal glycosides that are identified for the first time from the nature.
重楼甾体皂甙因其结构多样性和显著的生物活性而引起科学界的关注。在这项工作中，采用超高效液相色谱-串联四极杆飞行时间质谱联用（UPLC/Q-TOF MS）技术快速分离和鉴定云南重楼根茎提取物中的甾体皂甙。糖苷键和跨环裂解的碎片离子提供了丰富的结构信息，可用于鉴定苷元、糖的类型以及糖单元的连接顺序。基于精确质量信息、裂解特征和 21 个甾体皂甙标准品的 LC 保留时间，初步鉴定了 98 种成分，并提出了它们的结构，涵盖了 30 多种甾体苷元。这 98 种成分包括 22 对结构异构体和 40 种甾体糖苷，其中 40 种甾体糖苷是首次从自然界中鉴定出来的。
