从印度中部医院废水分离的大肠杆菌中，鉴定出了具有广谱β-内酰胺酶和喹诺酮类耐药基因。
Identification of extended-spectrum β-lactamase and quinolone resistance genes in Escherichia coli isolated from hospital wastewater from central India.
机构信息
Department of Public Health and Environment, R. D. Gardi Medical College, Agar Road, Ujjain, India.
出版信息
J Antimicrob Chemother. 2012 Apr;67(4):857-9. doi: 10.1093/jac/dkr564. Epub 2012 Jan 19.
OBJECTIVES
To investigate the presence of antibiotic resistance genes (ARGs), related to commonly used β-lactams and quinolones, in Escherichia coli present in hospital wastewater in central India.
METHODS
Cefotaxime- and ciprofloxacin-resistant E. coli isolates from hospital-associated wastewater samples were collected from two tertiary care hospitals in the Ujjain district of India during 2008-09. The presence of bla(CTX-M), bla(TEM,) bla(SHV), qnrA, qnrB, qnrS, aac(6')-Ib-cr and qepA genes was detected by PCR and sequencing.
RESULTS
Twenty-five E. coli isolates were extended-spectrum β-lactamase (ESBL) positive. bla(CTX-M-15) and bla(TEM-1) genes were identified in 21 and 16 ESBL-positive isolates by PCR, respectively. Amongst 30 fluoroquinolone-resistant E. coli isolates, aac(6')-Ib-cr was detected in 27 isolates, qnrA in 1 isolate, qnrB in 2 isolates and qepA in 3 isolates.
CONCLUSIONS
bla(CTX-M-15,) bla(TEM-1), aac(6')-Ib-cr, qnrA, qnrB and qepA genes are present in E. coli occurring in hospital wastewater in central India.
目的
调查存在于印度中部医院废水中的大肠杆菌中与常用β-内酰胺类和喹诺酮类相关的抗生素耐药基因（ARGs）。
方法
2008-2009 年，从印度乌贾因地区的两家三级护理医院采集与医院相关的废水样本中分离出头孢他啶和环丙沙星耐药的大肠杆菌。通过 PCR 和测序检测 bla(CTX-M)、bla(TEM)、bla(SHV)、qnrA、qnrB、qnrS、aac(6')-Ib-cr 和 qepA 基因的存在。
结果
25 株大肠杆菌为超广谱β-内酰胺酶（ESBL）阳性。通过 PCR 分别鉴定出 21 株和 16 株 ESBL 阳性菌中 bla(CTX-M-15)和 bla(TEM-1)基因。在 30 株氟喹诺酮耐药的大肠杆菌中，27 株检测到 aac(6')-Ib-cr，1 株检测到 qnrA，2 株检测到 qnrB，3 株检测到 qepA。
结论
bla(CTX-M-15)、bla(TEM-1)、aac(6')-Ib-cr、qnrA、qnrB 和 qepA 基因存在于印度中部医院废水中的大肠杆菌中。
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