Harrisvaccines, Inc., Ames, IA 50010, USA.
Vaccine. 2012 Mar 2;30(11):1944-50. doi: 10.1016/j.vaccine.2012.01.030. Epub 2012 Jan 23.
A single-cycle, propagation-defective replicon particle (RP) vaccine expressing a swine influenza virus hemagglutinin (HA) gene was constructed and evaluated in several different animal studies. Studies done in both the intended host (pigs) and non-host (mice) species demonstrated that the RP vaccine is not shed or spread by vaccinated animals to comingled cohorts, nor does it revert to virulence following vaccination. In addition, vaccinated pigs develop both specific humoral and IFN-γ immune responses, and young pigs are protected against homologous influenza virus challenge.
构建并评估了一种单周期、传播缺陷的复制子颗粒（RP）疫苗，该疫苗表达了猪流感病毒血凝素（HA）基因。在不同的动物研究中进行的研究表明，RP 疫苗不会在接种动物中传播或传播给混合接种的动物群，也不会在接种后恢复毒力。此外，接种疫苗的猪会产生特异性体液和 IFN-γ 免疫应答，并且幼猪可以免受同源流感病毒的攻击。
