比较两种非侵入性方法在人体和动物皮肤中测定类胡萝卜素的效果：拉曼光谱与反射光谱。

Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy.

机构信息

Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology-CCP, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.

出版信息

J Biophotonics. 2012 Jul;5(7):550-8. doi: 10.1002/jbio.201100080. Epub 2012 Jan 23.

DOI:10.1002/jbio.201100080

PMID:22271669

Abstract

Based on compelling in vivo and in vitro studies on human skin, carotenoids are thought to be of great interest as powerful antioxidants acting to prevent free-radical-induced damages, including premature skin ageing and the development of skin diseases such as cancer. Among the available techniques that are suitable for noninvasive determination of carotenoids in human skin, are resonance Raman spectroscopy (RRS) and reflection spectroscopy (RS). For RS, a LED-based miniaturized spectroscopic system (MSS) was developed for noninvasive measurement of carotenoids in human skin. The optimization and subsequent calibration of the MSS was performed with the use of RRS. A strong correlation between the carotenoid concentration determined by the RS and for the RRS system was achieved for human skin in vivo (R = 0.88) and for bovine udder skin in vitro (R = 0.81).

摘要

基于对人体皮肤的强有力的体内和体外研究，类胡萝卜素被认为是非常有趣的，因为它们作为强大的抗氧化剂，可以防止自由基引起的损伤，包括皮肤过早老化和皮肤疾病（如癌症）的发展。在适用于非侵入性测定人体皮肤中类胡萝卜素的现有技术中，有共振拉曼光谱（RRS）和反射光谱（RS）。对于 RS，开发了基于 LED 的小型化光谱系统（MSS），用于非侵入性测量人体皮肤中的类胡萝卜素。使用 RRS 对 MSS 进行了优化和后续校准。体内人体皮肤（R = 0.88）和体外牛乳房皮肤（R = 0.81）的 RS 确定的类胡萝卜素浓度与 RRS 系统之间存在很强的相关性。