Applied Mycology Unit, Food Technology Department, University of Lleida, XaRTA-UTPV, Rovira Roure 191, 25198 Lleida, Spain.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(5):799-808. doi: 10.1080/19440049.2011.644813. Epub 2012 Jan 24.
Fumonisin B₁ (FB₁) and B₂ (FB₂) are mycotoxins produced by Fusarium verticillioides and F. proliferatum and common contaminants of cereal crops. The objectives of this study were to (1) study the occurrence of fumonisins in Catalonia (north-eastern region of Spain) and (2) assess the exposure of the Catalonian population to these mycotoxins. Contamination data was provided by a wide survey where 928 individual samples were pooled to analyse 370 composite samples. Fumonisins were extracted and purified using immunoaffinity columns and determined by HPLC with fluorescence detection. The raw consumption data came from a nutritional study specifically designed to assess the dietary intake of the main foodstuffs related to fumonisin contamination for all population age groups. In addition, two specific groups were selected with respect to maize consumption: immigrants and celiac sufferers. Contamination and consumption data were combined by simulation using an essentially parametric-parametric (P-P) method. The P-P method draws sampling values from distribution functions fitted to consumption and contamination datasets. Moreover, to quantify the accuracy and reliability of the statistical estimates, we built related confidence intervals using a Pseudo-Parametric bootstrap method. The results of this study show that fumonisins are commonly found in some commodities on the Catalonian market, such as beer, corn snacks and ethnic foods; however, the values were well below the permitted maximum EU levels. The most exposed group were infants followed by immigrants but, in all cases, they were below the TDI of 2 µg/kg bw/day.
伏马菌素 B₁（FB₁）和 B₂（FB₂）是由串珠镰刀菌和多育镰刀菌产生的真菌毒素，是谷物作物的常见污染物。本研究的目的是：（1）研究伏马菌素在加泰罗尼亚（西班牙东北部地区）的发生情况；（2）评估加泰罗尼亚人群对这些真菌毒素的暴露情况。污染数据来自一项广泛的调查，其中汇集了 928 个个体样本，以分析 370 个复合样本。使用免疫亲和柱提取和纯化伏马菌素，并用 HPLC 荧光检测法测定。原始消费数据来自一项专门设计的营养研究，旨在评估与伏马菌素污染相关的主要食品的饮食摄入量，涵盖所有年龄段的人群。此外，根据玉米的消费情况，选择了两个特定的群体：移民和麸质过敏患者。通过模拟，使用基本的参数-参数（P-P）方法将污染和消费数据结合在一起。P-P 方法从拟合消费和污染数据集的分布函数中抽取抽样值。此外，为了量化统计估计的准确性和可靠性，我们使用伪参数引导法构建了相关的置信区间。这项研究的结果表明，伏马菌素在加泰罗尼亚市场的一些商品中很常见，如啤酒、玉米小吃和民族食品；然而，这些值远低于欧盟允许的最高限量。暴露最严重的群体是婴儿，其次是移民，但在所有情况下，其摄入量都低于 2µg/kg bw/day 的 TDI。
