启蒙时代：通过光操控神经元活动来挖掘大脑研究中的新机遇。
The age of enlightenment: evolving opportunities in brain research through optical manipulation of neuronal activity.
机构信息
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center Memphis, TN, USA.
出版信息
Front Syst Neurosci. 2011 Dec 7;5:95. doi: 10.3389/fnsys.2011.00095. eCollection 2011.
Abstract
Optical manipulation of neuronal activity has rapidly developed into the most powerful and widely used approach to study mechanisms related to neuronal connectivity over a range of scales. Since the early use of single site uncaging to map network connectivity, rapid technological development of light modulation techniques has added important new options, such as fast scanning photostimulation, massively parallel control of light stimuli, holographic uncaging, and two-photon stimulation techniques. Exciting new developments in optogenetics complement neurotransmitter uncaging techniques by providing cell-type specificity and in vivo usability, providing optical access to the neural substrates of behavior. Here we review the rapid evolution of methods for the optical manipulation of neuronal activity, emphasizing crucial recent developments.
摘要
光遗传学技术补充了神经递质光解技术，提供了细胞类型特异性和体内可用性，为行为的神经基础提供了光学通道。在这里，我们回顾了光操纵神经元活动方法的快速发展，强调了最近的关键进展。
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