Verdier M, Denis F, Leonard G, Sangare A, Patillaud S, Prince-David M, Essex M
Department of Bacteriology-Virology, Centre Hospitalier Universitaire Dupuytren, Limoges, France.
J Clin Microbiol. 1990 Sep;28(9):1988-93. doi: 10.1128/jcm.28.9.1988-1993.1990.
The effectiveness of four screening tests for detecting antibody to human T-cell leukemia virus type I (HTLV-I) was determined by using 2,700 African serum specimens. The tests studied were indirect immunofluorescence, particle agglutination from Fujirebio, and two enzyme immunoassays, one from Abbott Laboratories that used virus lysate from HUT 102 cells and the other from Cambridge BioScience Corp. that used an env recombinant protein. Positive and doubtful sera were confirmed by Western immunoblot and radioimmunoprecipitation assay with Food and Drug Administration seropositivity criteria. The best results were obtained with the two enzyme immunoassays, which were more sensitive (100 and 98.6% [Abbott and Cambridge, respectively]) and more specific (98.7 and 96.5%). Indirect immunofluorescence exhibited difficulties for reading and interpretation. With particle agglutination, prozone was observed for 9 of 78 HTLV-I-positive serum specimens. False-positives in any of the tests were not linked to cross-reactions with human immunodeficiency viruses. However, confirmation tests remain necessary for HTLV-I screening.
通过使用2700份非洲血清标本，确定了四种检测人类T细胞白血病病毒I型（HTLV-I）抗体的筛查试验的有效性。所研究的试验包括间接免疫荧光法、富士瑞必欧的颗粒凝集法以及两种酶免疫测定法，一种是雅培实验室采用HUT 102细胞病毒裂解物的酶免疫测定法，另一种是剑桥生物科学公司采用env重组蛋白的酶免疫测定法。根据美国食品药品监督管理局的血清阳性标准，通过蛋白质印迹法和放射免疫沉淀试验对阳性和可疑血清进行确认。两种酶免疫测定法获得了最佳结果，它们更敏感（分别为100%和98.6%[雅培和剑桥]）且更具特异性（分别为98.7%和96.5%）。间接免疫荧光法在判读和解释方面存在困难。在颗粒凝集法中，78份HTLV-I阳性血清标本中有9份出现前带现象。任何一项试验中的假阳性均与人类免疫缺陷病毒的交叉反应无关。然而，HTLV-I筛查仍需要进行确认试验。
