Department of Pharmacological Sciences, Università degli Studi di Milano, Milano, Italy.
Phytother Res. 2012 Oct;26(10):1569-71. doi: 10.1002/ptr.4600. Epub 2012 Feb 1.
The present work evaluates the effect of olive oil phenols on NF-κB activity in human gastric adenocarcinoma cells. The total phenol content was measured by the Folin Ciocalteu method, whereas the composition was assessed by LC-MS/MS analysis. Secoiridoids represented 71% and 83% of the Italian and Spanish extracts, respectively, phenol alcohols were in the range 9-13%. Ligustroside aglycone was the most abundant (37% and 46%, respectively, in the Italian and Spanish sample), and the concentration of flavonoids AP and LU was below 1%. Phenol extracts were assayed at 0.25-7.5 µg/mL, whereas single compounds were at 0.5-25 µM. Both the extracts inhibited the NF-κB driven transcription in a concentration-dependent manner: IC(50) for the Italian and the Spanish extract were 0.86 and 1.28 µg/mL, respectively. The IC(50) for individual compounds ranged from 4.5 to 13 µM. All the compounds under study inhibited nuclear translocation as well. The data suggest that consumption of extra-virgin olive oil may be beneficial for preventing the onset of gastric inflammation leading to more serious diseases.
本研究评估了橄榄油酚类化合物对人胃腺癌 NF-κB 活性的影响。采用 Folin-Ciocalteu 法测定总酚含量，采用 LC-MS/MS 分析评估其组成。意大利和西班牙提取物中分别有 71%和 83%为赛尔维达宁类，酚类醇的含量在 9-13%之间。橄榄苦苷配基分别为意大利和西班牙样本中的最丰富成分（分别占 37%和 46%），且类黄酮 AP 和 LU 的浓度低于 1%。酚类提取物的检测浓度为 0.25-7.5 µg/mL，而单一化合物的检测浓度为 0.5-25 µM。提取物均呈浓度依赖性抑制 NF-κB 驱动的转录：意大利和西班牙提取物的 IC50 分别为 0.86 和 1.28 µg/mL。研究中的所有化合物的 IC50 范围为 4.5-13 µM。所有研究中的化合物均能抑制核转位。这些数据表明，食用特级初榨橄榄油可能有助于预防导致更严重疾病的胃炎症的发生。
