Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, People's Republic of China.
Luminescence. 2013 Jan-Feb;28(1):56-62. doi: 10.1002/bio.2337. Epub 2012 Feb 2.
In this study, a sensitive and simple flow-injection chemiluminescence (CL) method was developed for the quantitative analysis of haemoglobin. The method is based on the ability of haemoglobin to enhance the CL signal generated by a H(2)O(2) -K(4) Fe(CN)(6) -fluorescein alkaline system enhanced by CdTe quantum dots. Under the optimized conditions, haemoglobin can be detected in concentration range 7.35 × 10(-9) -2.5 × 10(-6) mol/L, with a detection limit (3σ) of 1.8 × 10(-9) mol/L and a relative standard deviation (RSD; for 5 × 10(-7) mol/L haemoglobin) of 2.06% (n = 11). The present CL method was successfully applied for the determination of haemoglobin in three kinds of blood samples taken from an infant, an adult man, an adult woman and two reference samples. Compared with previous reports, the CL method described in this work is simple and rapid, with high sensitivity.
在这项研究中，开发了一种灵敏且简单的流动注射化学发光（CL）方法，用于血红蛋白的定量分析。该方法基于血红蛋白增强 H（2）O（2）-K（4）Fe（CN）（6）-荧光素碱性体系产生的 CL 信号的能力，该体系由 CdTe 量子点增强。在优化条件下，血红蛋白可在 7.35×10（-9）-2.5×10（-6）mol/L 的浓度范围内检测到，检测限（3σ）为 1.8×10（-9）mol/L，相对标准偏差（RSD；对于 5×10（-7）mol/L 的血红蛋白）为 2.06%（n = 11）。本 CL 法成功地用于测定取自婴儿、成年男性、成年女性和两种参考样品的三种血液样品中的血红蛋白。与以前的报道相比，本工作中描述的 CL 方法简单快速，具有高灵敏度。
