Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
J Clin Periodontol. 2012 May;39(5):417-24. doi: 10.1111/j.1600-051X.2012.01855.x. Epub 2012 Feb 6.
This study was undertaken to investigate the existence of a periodontopathic bacterium, Fusobacterium nucleatum, in chorionic tissues of pregnant women, and the effects of F. nucleatum on human chorion-derived cells.
Oral and chorionic tissue samples were collected from 24 high-risk pregnant women and 15 normal pregnant women. The presence of F. nucleatum in the samples was detected using polymerase chain reaction. Chorion-derived cells and Toll-like receptor (TLR)-2 or TLR-4 gene-silenced chorion-derived cells were stimulated with F. nucleatum lipopolysaccharide (LPS). Interleukin (IL)-6 and corticotrophin-releasing hormone (CRH) levels in the culture supernatants were measured using ELISA.
F. nucleatum was detected in all oral samples and seven chorionic tissues from the high-risk pregnant women, but was not detected in chorionic tissues from the normal pregnant women. F. nucleatum LPS significantly increased IL-6 and CRH secretion by chorion-derived cells. The F. nucleatum LPS-induced IL-6 and CRH levels were significantly reduced in TLR-2 or TLR-4 gene-silenced chorion-derived cells.
We suggest that F. nucleatum is detected in chorionic tissues of high-risk pregnant women, but not in chorionic tissues of normal pregnant women, and that F. nucleatum induces IL-6 and CRH production via both TLR-2 and TLR-4 in chorion-derived cells.
本研究旨在调查牙周病病原菌核梭杆菌（Fusobacterium nucleatum）是否存在于孕妇的绒毛组织中，以及 F. nucleatum 对人绒毛膜来源细胞的影响。
采集 24 例高危孕妇和 15 例正常孕妇的口腔和绒毛组织样本。采用聚合酶链反应（PCR）检测样本中 F. nucleatum 的存在情况。用 F. nucleatum 脂多糖（LPS）刺激绒毛膜来源细胞和 Toll 样受体（TLR）-2 或 TLR-4 基因沉默的绒毛膜来源细胞。采用 ELISA 法检测培养上清液中白细胞介素（IL）-6 和促肾上腺皮质激素释放激素（CRH）的水平。
F. nucleatum 在所有口腔样本和 24 例高危孕妇的 7 份绒毛组织中均被检出，但在 15 例正常孕妇的绒毛组织中未被检出。F. nucleatum LPS 显著增加了绒毛膜来源细胞分泌的 IL-6 和 CRH。TLR-2 或 TLR-4 基因沉默的绒毛膜来源细胞中，F. nucleatum LPS 诱导的 IL-6 和 CRH 水平显著降低。
我们认为，F. nucleatum 存在于高危孕妇的绒毛组织中，但不存在于正常孕妇的绒毛组织中，并且 F. nucleatum 通过 TLR-2 和 TLR-4 诱导绒毛膜来源细胞产生 IL-6 和 CRH。
