通过质谱法对前列腺炎患者精浆蛋白质组进行表征。

BACKGROUND

Prostatitis is an inflammation of the prostate gland which affects approximately 10% of men. Despite its frequency, diagnosing prostatitis and monitoring patient response to treatment remains frustrating. As the prostate contributes a substantial percentage of proteins to seminal plasma, we hypothesized that a protein biomarker of prostatitis might be found by comparing the seminal plasma proteome of patients with and without prostatitis.

RESULTS

Using mass spectrometry, we identified 1708 proteins in the pooled seminal plasma of 5 prostatitis patients. Comparing this list to a previously published list of seminal plasma proteins in the pooled seminal plasma of 5 healthy, fertile controls yielded 1464 proteins in common, 413 found only in the control group, and 254 found only in the prostatitis group. Applying a set of criteria to this dataset, we generated a high-confidence list of 59 candidate prostatitis biomarkers, 33 of which were significantly increased in prostatitis as compared to control, and 26 of which were decreased. The candidates were analyzed using Gene Ontology and Ingenuity Pathway analysis to delineate their subcellular localizations and functions.

CONCLUSIONS

Thus, in this study, we identified 59 putative biomarkers in seminal plasma that need further validation for diagnosis and monitoring of prostatitis.

背景

前列腺炎是一种前列腺炎症，影响约 10%的男性。尽管其发病率很高，但前列腺炎的诊断和监测患者对治疗的反应仍然令人沮丧。由于前列腺为精液提供了很大比例的蛋白质，我们假设通过比较前列腺炎患者和健康、有生育能力的对照组的精液蛋白质组，可以找到前列腺炎的蛋白质生物标志物。

结果

我们使用质谱法在 5 名前列腺炎患者的 pooled 精液中鉴定出 1708 种蛋白质。将这份清单与之前在 5 名健康、有生育能力的对照组 pooled 精液中发表的精液蛋白质清单进行比较，得到了 1464 种共同的蛋白质，413 种仅在对照组中发现，254 种仅在前列腺炎组中发现。应用一组标准对该数据集进行分析，我们生成了一份有 59 种候选前列腺炎生物标志物的高可信度清单，其中 33 种在前列腺炎中明显增加，26 种减少。对候选物进行了 Gene Ontology 和 Ingenuity Pathway 分析，以描绘它们的亚细胞定位和功能。