Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
Parasitology. 2012 Jun;139(7):904-14. doi: 10.1017/S0031182012000030. Epub 2012 Feb 8.
The sunbleak (Leucaspius delineatus), a cyprinid fish native to continental Europe and now established in the UK, is experiencing population decline which appears to be linked to the spread of the invasive Asian cyprinid (Pseudorasbora parva). A population of sunbleak in the UK has previously been identified as infected with S. destruens at low prevalence. Because Sphaerothaecum destruens has, on occasion, caused severe disease in cultured and wild salmonids the aim of this work was to establish laboratory cultures of S. destruens from sunbleak in the UK and use these cultures in challenge experiments to determine if the UK isolate of S. destruens from cyprinid species is a potential threat to Atlantic salmon (Salmo salar). The first isolation and culture of S. destruens in the UK and from a cyprinid species is described. Cultured S. destruens spores from sunbleak are infective to EPC, CHSE and FHM cells, replicating most rapidly in FHM and EPC cells. Spores can be induced to zoosporulate in water forming motile, uni-flagellated zoospores. Challenge experiments indicated the spores are able to replicate and disperse in Atlantic salmon and are associated with increased mortality (up to 90%) when injected intraperitonealy.
太阳鲈（Leucaspius delineatus），一种原产于欧洲大陆的鲤科鱼类，现已在英国定居，其数量正在减少，这似乎与入侵的亚洲鲤科鱼类（Pseudorasbora parva）的传播有关。英国的一个太阳鲈种群以前被发现感染了低流行率的 Sphaerothaecum destruens。因为 Sphaerothaecum destruens 偶尔会导致养殖和野生鲑鱼严重发病，所以本工作的目的是从英国的太阳鲈中分离 S. destruens 并建立实验室培养物，然后使用这些培养物进行挑战实验，以确定来自鲤科鱼类的 S. destruens 英国分离株是否对大西洋鲑（Salmo salar）构成潜在威胁。本文描述了 S. destruens 在英国的首次分离和培养，也是首次从鲤科鱼类中分离和培养。从太阳鲈中分离培养的 S. destruens 孢子对 EPC、CHSE 和 FHM 细胞具有感染性，在 FHM 和 EPC 细胞中复制最快。孢子可以在水中诱导形成游动的、单鞭毛的游动孢子进行zoosporulation。挑战实验表明，孢子能够在大西洋鲑中复制和扩散，并在腹腔内注射时导致死亡率增加（高达 90%）。
