Nahrevanian Hossein, Sheykhkanlooye Milan Bayram, Kazemi Masoud, Hajhosseini Reza, Soleymani Mashhadi Soudeh, Nahrevanian Shahab
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Malar Res Treat. 2012;2012:727032. doi: 10.1155/2012/727032. Epub 2012 Jan 23.
The aim of this study is pharmacochemistry of Iranian flora Artemisia sieberi and its antimalarial effects on Plasmodium berghei in vivo. This is the first application of A. sieberi for treatment of murine malaria. A. sieberi were collected at flowering stage from the Khorassan and Semnan provinces of Iran; the aerial parts were air-dried at room temperature and then powdered. The powder was macerated in methanol, filtered with Bokhner hopper and solvent was separated in rotary evaporator. Total herbal extract was subsequently processed for ether and chloroform extracts preparation. The toxicity of herbal extract was assessed on naive NMRI mice with high, average and low doses; then pathophysiological signs were assessed. Finally, the antimalarial efficacy was investigated on two groups of Plasmodium berghei infected mice. Percentage of parasitaemia and pathophysiology were also evaluated. The results of this assessment showed no toxicity even by high concentration of herbal extract. A significant reduction in percentage of parasitaemia was observed; no alterations of hepatosplenomegaly and body weight were indicated in study group. A. sieberi extracts showed antimalarial effects against murine malaria with some efficacies on reducing pathophysiology. However, there is requirement to find the major component of this herbal extract by further studies.
本研究旨在探讨伊朗植物西伯蒿的药物化学及其对体内伯氏疟原虫的抗疟作用。这是西伯蒿首次应用于治疗鼠疟。西伯蒿于花期从伊朗霍拉桑省和塞姆南省采集；地上部分在室温下风干，然后粉碎。将粉末用甲醇浸渍，用布氏漏斗过滤，溶剂在旋转蒸发仪中分离。随后对总草药提取物进行处理以制备乙醚和氯仿提取物。用高、中、低剂量对未接触过药物的NMRI小鼠评估草药提取物的毒性；然后评估病理生理体征。最后，对两组感染伯氏疟原虫的小鼠研究其抗疟效果。还评估了疟原虫血症百分比和病理生理学。该评估结果显示，即使是高浓度的草药提取物也没有毒性。观察到疟原虫血症百分比显著降低；研究组未显示肝脾肿大和体重有变化。西伯蒿提取物对鼠疟显示出抗疟作用，对减轻病理生理学有一定效果。然而，需要通过进一步研究找到这种草药提取物的主要成分。
