Grace Nirmala J, Narendhirakannan R T
Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Karunya Nagar, Coimbatore, India.
Asian Pac J Cancer Prev. 2011;12(10):2689-95.
Cervical cancer is the second common type of cancer among women worldwide, with the human papillomavirus (HPV) recognized as the major causative agent. The HPV 16/18 prevalance in cervical cancer patients from the Trichy and Coimbatore districts of Tamil Nadu state, India, was evaluated in addition to an assessment of oxidative stress and antioxidant status. MDA, GSH, GPx, GST, SOD, vitamin C and vitamin E were estimated in the plasma and erythrocytes of the twenty patients and an equal number of age matched normal subjects as controls. 119 paraffin embedded tissue samples were collected to perform DNA extraction and genotyping of HPV 16/18 using specific primers. Plasma and erythrocyte TBARS level was significantly elevated in the cervical cancer patients compared to normal. It was observed that SOD, GPx, GSH levels in the erythrocyte and plasma was significantly lower in cervical cancer patients, as well as GST and Vitamins E and C levels in the plasma and catalase enzyme levels in the erythrocytes. Genotyping showed 57% positive for HPV16 and 18% for HPV18, indicating that vaccination against these two will effectively reduce the burden associated with the disease. These findings suggest possible use of antioxidant supplementation as prophylactic agents for prevention and treatment of cervical cancer.
宫颈癌是全球女性中第二常见的癌症类型，人乳头瘤病毒（HPV）被认为是主要致病因素。除了评估氧化应激和抗氧化状态外，还对印度泰米尔纳德邦蒂鲁吉拉伯利和哥印拜陀地区宫颈癌患者的HPV 16/18感染率进行了评估。对20名患者以及同等数量年龄匹配的正常受试者作为对照的血浆和红细胞中的丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）、超氧化物歧化酶（SOD）、维生素C和维生素E进行了测定。收集了119份石蜡包埋组织样本，使用特异性引物进行HPV 16/18的DNA提取和基因分型。与正常组相比，宫颈癌患者血浆和红细胞中的硫代巴比妥酸反应物（TBARS）水平显著升高。观察到宫颈癌患者红细胞和血浆中的SOD、GPx、GSH水平显著降低，血浆中的GST、维生素E和C水平以及红细胞中的过氧化氢酶水平也显著降低。基因分型显示HPV16阳性率为57%，HPV18为18%，这表明针对这两种病毒的疫苗接种将有效减轻该疾病的负担。这些发现表明抗氧化剂补充剂可能作为预防和治疗宫颈癌的预防剂。
