Jelić Marija, Mandić Aljoša, Kladar Nebojša, Sudji Jan, Božin Biljana, Srdjenović Branislava
University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia.
Oncology Institute of Vojvodina, Sremska Kamenica, Serbia.
J Med Biochem. 2018 Jul 1;37(3):336-345. doi: 10.1515/jomb-2017-0053. eCollection 2018 Jul.
Oxidative stress has been associated with cervical cancer. Our aim was to examine lipid peroxidation and the extent of oxidative stress in women diagnosed with different stages of cervical cancer in order to evaluate its potential role in the evolution of cancer.
We measured the concentration of thiobarbituric acid reactive substances, activities of antioxidative enzymes and 8-hydroxy-2-deoxyguanosine in 153 subjects. Enzymatic activity as well as TBARS concentration were measured spectrophotometrically, while 8-OHdG was determined by gas chromatography-mass spectrometry. PPatients were categorized: group II H-SIL; group III FIGO Ia-Ib and group IV FIGO IIa-IV.
Our results showed highly significant increase in the level of lipid peroxidation in group IV when com pared to the control group, group II and group III (p<0.001). Activity of superoxide dismutase was also significantly higher in group IV when compared to control group (p<0.01), group II (p<0.01) and group III (p<0.05). Activity of catalase was also significantly higher in group IV when compared to control group (p<0.005), group II (p<0.005) and group III (p<0.05). Activity of glutathione-S-transferase was also significantly higher in group IV when compared to control group (p<0.05), group II (p<0.05) and group III (p<0.05). Activities of glutathione peroxidase and glutathione reductase showed no significant differences among the groups. Level of 8-OHdG was significantly higher in group IV than in the other groups (p<0.01).
It can be concluded that oxidative stress is possibly involved in the pathogenesis of cervical cancer, demonstrated by increased lipid peroxidation and an altered antioxidant defense system and higher levels of 8-OHdG.
氧化应激与宫颈癌有关。我们的目的是检测被诊断为不同阶段宫颈癌的女性的脂质过氧化和氧化应激程度，以评估其在癌症发展中的潜在作用。
我们测量了153名受试者中硫代巴比妥酸反应性物质的浓度、抗氧化酶活性和8-羟基-2-脱氧鸟苷。酶活性以及硫代巴比妥酸反应物浓度通过分光光度法测量，而8-羟基脱氧鸟苷通过气相色谱-质谱法测定。患者被分为：II组高级别鳞状上皮内病变；III组国际妇产科联盟（FIGO）Ia-Ib期；IV组FIGO IIa-IV期。
我们的结果显示，与对照组、II组和III组相比，IV组的脂质过氧化水平显著升高（p<0.001）。与对照组（p<0.01）、II组（p<0.01）和III组（p<0.05）相比，IV组超氧化物歧化酶的活性也显著更高。与对照组（p<0.005）、II组（p<0.005）和III组（p<0.05）相比，IV组过氧化氢酶的活性也显著更高。与对照组（p<0.05）、II组（p<0.05）和III组（p<0.05）相比，IV组谷胱甘肽-S-转移酶的活性也显著更高。谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性在各组之间没有显著差异。IV组的8-羟基脱氧鸟苷水平显著高于其他组（p<0.01）。
可以得出结论，氧化应激可能参与宫颈癌的发病机制，脂质过氧化增加、抗氧化防御系统改变以及8-羟基脱氧鸟苷水平升高证明了这一点。
