使用SWISS-MODEL工作区和蛋白质模型门户进行自动化蛋白质结构建模。
Automated protein structure modeling with SWISS-MODEL Workspace and the Protein Model Portal.
作者信息
Bordoli Lorenza, Schwede Torsten
机构信息
SIB Swiss Institute of Bioinformatics, Biozentrum University of Basel, Basel, Switzerland.
出版信息
Methods Mol Biol. 2012;857:107-36. doi: 10.1007/978-1-61779-588-6_5.
Abstract
Comparative protein structure modeling is a computational approach to build three-dimensional structural models for proteins using experimental structures of related protein family members as templates. Regular blind assessments of modeling accuracy have demonstrated that comparative protein structure modeling is currently the most reliable technique to model protein structures. Homology models are often sufficiently accurate to substitute for experimental structures in a wide variety of applications. Since the usefulness of a model for specific application is determined by its accuracy, model quality estimation is an essential component of protein structure prediction. Comparative protein modeling has become a routine approach in many areas of life science research since fully automated modeling systems allow also nonexperts to build reliable models. In this chapter, we describe practical approaches for automated protein structure modeling with SWISS-MODEL Workspace and the Protein Model Portal.
摘要
比较蛋白质结构建模是一种计算方法，它以相关蛋白质家族成员的实验结构为模板，构建蛋白质的三维结构模型。对建模准确性的定期盲评表明，比较蛋白质结构建模是目前模拟蛋白质结构最可靠的技术。同源模型通常足够准确，可在多种应用中替代实验结构。由于模型在特定应用中的有用性取决于其准确性，因此模型质量评估是蛋白质结构预测的重要组成部分。自从全自动建模系统使非专业人员也能构建可靠模型以来，比较蛋白质建模已成为生命科学研究许多领域的常规方法。在本章中，我们将描述使用SWISS-MODEL工作区和蛋白质模型门户进行自动蛋白质结构建模的实用方法。
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