Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, P.O. Box 77000, Port Elizabeth 6031, South Africa.
Phytomedicine. 2012 Apr 15;19(6):488-93. doi: 10.1016/j.phymed.2011.12.008. Epub 2012 Feb 10.
Marrubiin and an organic extract of Leonotis leonurus were tested in vitro and in vivo for their antidiabetic and anti-inflammatory activities.
INS-1 cells were cultured under normo- and hyperglycemic conditions conditions. An in vivo animal model confirmed the biological activities of marrubiin and the organic extract observed in the studies in vitro.
The stimulatory index of INS-1 cells cultured under hyperglycemic conditions was significantly increased in cells exposed to the organic extract and marrubiin, relative to the hyperglycaemic conditions. Insulin and glucose transporter-2 gene expressions were significantly increased by the organic extract and marrubiin. Similarly, the extract and marrubiin resulted in an increase in respiratory rate and mitochondrial membrane potential under hyperglycaemic conditions. Marrubiin increased insulin secretion, HDL-cholesterol, while it normalized total cholesterol, LDL-cholesterol, atherogenic index, IL-1β and IL-6 levels in an obese rat model.
The results provide evidence that marrubiin, a constituent of Leonotis leonurus, alleviates diabetic symptoms.
对毛蕊花糖苷和 Leonotis leonurus 的有机提取物进行体内和体外试验，以评估其抗糖尿病和抗炎活性。
在正常血糖和高血糖条件下培养 INS-1 细胞。体内动物模型证实了毛蕊花糖苷和有机提取物在体外研究中观察到的生物活性。
与高血糖条件相比，暴露于有机提取物和毛蕊花糖苷的高血糖条件下培养的 INS-1 细胞的刺激指数显著增加。有机提取物和毛蕊花糖苷显著增加了胰岛素和葡萄糖转运蛋白-2 基因的表达。同样，在高血糖条件下，提取物和毛蕊花糖苷导致呼吸率和线粒体膜电位增加。毛蕊花糖苷可增加胰岛素分泌和 HDL 胆固醇，同时可使肥胖大鼠模型中的总胆固醇、LDL 胆固醇、致动脉粥样硬化指数、IL-1β 和 IL-6 水平正常化。
结果表明，Leonotis leonurus 的一种成分毛蕊花糖苷可缓解糖尿病症状。
