Rezgui Marwa, Basma Mabrouk, Neng Nuno, Nogueira José Manuel, Bettaieb Ben-Kaab Leila, Machado Araújo Maria Eduarda
Faculty of Sciences of Tunisia, University Tunis El Manar, Tunis 2092, Tunisia.
Centro de Química Estrutural, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande Ed. C8, 1749-016 Lisboa, Portugal.
Foods. 2021 Nov 19;10(11):2864. doi: 10.3390/foods10112864.
L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and -carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.
夏至草（L.），俗称夏至草属植物，是一种分布广泛且广为人知的植物，用于啤酒酿造厂，在突尼斯也是一种传统药物。在本研究中，使用三种测定方法（铁还原能力、自由基清除活性和β-胡萝卜素-亚油酸漂白测定）以及总酚含量，对从五个不同地点采集的植物甲醇提取物的抗氧化活性进行了研究。还对该植物的矿物质组成进行了研究，涉及以下元素：铁、镁、钙、铜、锌、锰、钾以及三种重金属镍、铅和镉。通过核磁共振对样品中的主要生物活性二萜内酯绵马酸进行了定量。通过水蒸馏法获得精油，并对其自由基清除活性进行了研究。评估了精油对卤虫（盐水虾幼虫）的毒性。精油表现出较弱的自由基清除活性和低毒性。从五个不同地点获得的数据表明，收获地点对抗氧化活性以及总酚和绵马酸含量有很大影响。样品中的金属含量因收获地点而异，但钙、镁和钾的含量始终很高。
