Morishita Yoshiyuki, Watanabe Minami, Hirahara Ichiro, Akimoto Tetsu, Muto Shigeaki, Kusano Eiji
Division of Nephrology, Department of Medicine, Jichi Medical University, Tochigi, Japan.
Int J Nephrol Renovasc Dis. 2012;5:9-14. doi: 10.2147/IJNRD.S27553. Epub 2011 Dec 29.
Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage. Therefore, the monitoring and estimation of peritoneal function are important in PD patients. Oxidative stress has been implicated as one possible mechanism of peritoneal membrane damage. The aim of this study was to evaluate the association between an oxidative stress marker, 8-hydroxydeoxyguanosine (8-OHdG), and peritoneal damage in PD patients.
The authors evaluated 8-OHdG in drained dialysate by enzyme immunoassay to investigate the association between 8-OHdG and solute transport rate estimated by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in 45 samples from 28 PD patients.
The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05). The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.
The level of 8-OHdG in drained dialysate may be a useful novel marker of peritoneal damage in PD.
腹膜透析（PD）是一种成功的肾脏替代疗法；然而，长期进行腹膜透析会导致腹膜结构和功能受损。因此，对腹膜功能的监测和评估在腹膜透析患者中很重要。氧化应激被认为是腹膜损伤的一种可能机制。本研究的目的是评估氧化应激标志物8-羟基脱氧鸟苷（8-OHdG）与腹膜透析患者腹膜损伤之间的关联。
作者通过酶免疫测定法评估引流透析液中的8-OHdG，以研究28例腹膜透析患者的45份样本中8-OHdG与通过腹膜平衡试验估计的溶质转运率以及基质金属蛋白酶-2（MMP-2）水平之间的关联。
8-OHdG水平与透析液：血浆肌酐比值显著相关（r = 0.463，P < 0.05），与D/D0葡萄糖（其中D是注射后4小时获得的腹膜流出液中的葡萄糖水平，D0是注射后立即获得的葡萄糖水平）显著负相关（r = -0.474，P < 0.05）。8-OHdG水平也与MMP-2水平显著相关（r = 0.551，P < 0.05），但与受试者年龄、腹膜透析持续时间或血压无关。
引流透析液中8-OHdG的水平可能是腹膜透析患者腹膜损伤的一种有用的新标志物。
