Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
J Anal Toxicol. 2012 Mar;36(2):136-40. doi: 10.1093/jat/bkr016.
The use of oral fluid (OF) as an alternative matrix for the detection of drugs of abuse has increased over the last decade, leading to the need for a rapid, simple, and reliable on-site OF testing device. Four on-site OF drug testing devices (Dräger DrugTest 5000, Cozart DDS, Mavand Rapid STAT, and Innovacon OrAlert) were evaluated on 408 volunteers at drug treatment centers. UPLC-MS-MS results were used as reference to determine sensitivity, specificity and accuracy for each device, applying Belgian legal confirmation cutoffs for benzoylecgonine, cocaine, and THC (10 ng/mL); morphine and 6-acetylmorphine (5 ng/mL); and amphetamine and 3,4-methylenedioxymethylamphetamine (25 ng/mL). Sensitivity for cocaine was 50%, 50%, 27%, and 11% for DrugTest, OrAlert, Rapid STAT, and DDS 806, respectively. For opiates, sensitivities were 84%, 73%, 77%, and 65%, respectively. For THC, the sensitivities were 81%, 23%, 43%, and 28%, respectively. For amphetamines, the sensitivities were 75%, 33%, 17%, and 67%, respectively. Specificity was >88% for opiates and THC, > 90% for amphetamines, and > 97% for cocaine. All tests showed good specificity. DrugTest had the highest sensitivity, although it was still low for some analytes.
在过去十年中，人们越来越多地使用口服液（OF）作为替代基质来检测滥用药物，这导致了对快速、简单和可靠的现场 OF 检测设备的需求。在药物治疗中心，对 408 名志愿者评估了四种现场 OF 药物检测设备（Dräger DrugTest 5000、Cozart DDS、Mavand Rapid STAT 和 Innovacon OrAlert）。UPLC-MS-MS 结果被用作参考，以确定每种设备的灵敏度、特异性和准确性，为苯甲酰古柯碱、可卡因和四氢大麻酚（10ng/mL）；吗啡和 6-乙酰吗啡（5ng/mL）；以及苯丙胺和 3,4-亚甲基二氧甲基苯丙胺（25ng/mL）应用比利时法律确认的截止值。可卡因的灵敏度分别为 DrugTest、OrAlert、Rapid STAT 和 DDS 806 的 50%、50%、27%和 11%。对于阿片类药物，灵敏度分别为 84%、73%、77%和 65%。对于四氢大麻酚，灵敏度分别为 81%、23%、43%和 28%。对于苯丙胺，灵敏度分别为 75%、33%、17%和 67%。特异性>88%用于阿片类药物和四氢大麻酚，>90%用于苯丙胺，>97%用于可卡因。所有测试均表现出良好的特异性。DrugTest 的灵敏度最高，尽管对于某些分析物而言，其灵敏度仍然较低。
