Yoon Ki Nam, Alam Nuhu, Shim Mi Ja, Lee Tae Soo
Division of Life Sciences, University of Incheon, Incheon 406-840, Republic of Korea.
Int J Med Mushrooms. 2012;14(1):27-36. doi: 10.1615/intjmedmushr.v14.i1.30.
The present study was conducted to investigate dietary supplementation of Pleurotus salmoneostramineus fruiting bodies on biochemical and histological effects in hyper- and normocholesterolemic rats. Six-week-old female Sprague-Dawley albino rats were divided into three groups of 10 rats each. Feeding of diet containing a 5% powder of the fruiting bodies of P. salmoneostramineus in hypercholesterolemic rats reduced plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, phospholipids, and LDL/HDL ratio by 22.55, 51.38, 69.23, 29.67, 16.61, and 65.31%, respectively. The mushroom also significantly reduced body weight in hypercholesterolemic rats. Moreover, it had no adverse effects on plasma albumin, total bilirubin, direct bilirubin, creatinine, blood urea nitrogen, uric acid, glucose, total protein, calcium, sodium, potassium, chloride, inorganic phosphate, magnesium, and enzyme profiles. Feeding mushroom increased total lipid and cholesterol excretion in feces. The plasma lipoprotein fraction, separated by agarose gel electrophoresis, indicated that P. salmoneostramineus significantly reduced plasma β and pre-β-lipoprotein, while it increased α-lipoprotein. A histological study of liver tissues by conventional hematoxylin-eosin and oil red O staining showed normal in mushroom feed hypercholesterolemic rat. This study suggests that the P. salmoneostramineus diet supplement provided health benefits by acting on the atherogenic lipid profile in hypercholesterolemic rats.
本研究旨在探讨膳食补充鲑鱼侧耳子实体对高胆固醇血症和正常胆固醇血症大鼠的生化及组织学影响。六周龄雌性斯普拉格 - 道利白化大鼠被分为三组，每组10只。在高胆固醇血症大鼠中喂食含有5%鲑鱼侧耳子实体粉末的饲料，可使血浆总胆固醇、甘油三酯、低密度脂蛋白、总脂质、磷脂和低密度脂蛋白/高密度脂蛋白比值分别降低22.55%、51.38%、69.23%、29.67%、16.61%和65.31%。该蘑菇还显著降低了高胆固醇血症大鼠的体重。此外，它对血浆白蛋白、总胆红素、直接胆红素、肌酐、血尿素氮、尿酸、葡萄糖、总蛋白、钙、钠、钾、氯、无机磷酸盐、镁和酶谱没有不良影响。喂食蘑菇可增加粪便中总脂质和胆固醇的排泄。通过琼脂糖凝胶电泳分离的血浆脂蛋白组分表明，鲑鱼侧耳显著降低了血浆β和前β脂蛋白，同时增加了α脂蛋白。通过传统苏木精 - 伊红和油红O染色对肝脏组织进行的组织学研究表明，喂食蘑菇的高胆固醇血症大鼠肝脏组织正常。本研究表明，膳食补充鲑鱼侧耳可通过作用于高胆固醇血症大鼠的致动脉粥样硬化脂质谱而带来健康益处。
