Alam Nuhu, Yoon Ki Nam, Lee Tae Soo
Division of Life Sciences, University of Incheon, Incheon, Republic of Korea; Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh.
J Res Med Sci. 2011 Jun;16(6):776-86.
Pleurotus ferulae is an edible mushroom has been widely used for nutritional and medicinal purposes. Irrespective of the medicinal importance or therapeutic potentials of P. ferulae, there have not been studies on anti-hyperlipidemic properties. Therefore, the present study investigates the effects of dietary P. ferulae fruiting bodies on plasma and feces biochemical and on the liver histological status in hypercholesterolemic rats.
Six weeks old female Sprague-Dawley albino rats were divided into three groups of 10 rats each. Then biochemical and histological examinations were performed.
Feeding of a diet containing 5% P. ferulae fruiting bodies to hypercholesterolemic rat reduced plasma total cholesterol, triglyceride, low-density lipoprotein (LDL), total lipid, phospholipids, and LDL/high-density lipoprotein ratio by 30.02, 49.31, 71.15, 30.23, 21.93, and 65.31%, respectively. Mushroom also significantly reduced body weight in hypercholesterolemic rats. However, it had no adverse effects on plasma albumin, total bilirubin, direct bilirubin, creatinin, blood urea nitrogen, uric acid, glucose, total protein, calcium, sodium, potassium, chloride, inorganic phosphate, magnesium, and enzyme profiles. Feeding mushroom increased total lipid and cholesterol excretion in feces. The plasma lipoprotein fraction, separated by agarose gel electrophoresis, indicated that P. ferulae significantly reduced plasma β and pre-β-lipoprotein, while increased the α-lipoprotein. A histological study of hepatic cells by conventional hematoxylin-eosin and oil red O staining showed normal findings for mushroom-fed hypercholesterolemic rats.
The present study suggests that 5% P. ferulae diet supplement provides health benefits, at least partially, by acting on the atherogenic lipid profile in hypercholesterolemic rats.
阿魏侧耳是一种食用菌，已被广泛用于营养和药用目的。尽管阿魏侧耳具有药用价值或治疗潜力，但尚未有关于其抗高血脂特性的研究。因此，本研究调查了日粮中添加阿魏侧耳子实体对高胆固醇血症大鼠血浆和粪便生化指标以及肝脏组织学状态的影响。
将六周龄雌性斯普拉格-道利白化大鼠分为三组，每组10只。然后进行生化和组织学检查。
给高胆固醇血症大鼠喂食含5%阿魏侧耳子实体的日粮，可使血浆总胆固醇、甘油三酯、低密度脂蛋白（LDL）、总脂质、磷脂以及LDL/高密度脂蛋白比值分别降低30.02%、49.31%、71.15%、30.23%、21.93%和65.31%。该蘑菇还能显著降低高胆固醇血症大鼠的体重。然而，它对血浆白蛋白、总胆红素、直接胆红素、肌酐、血尿素氮、尿酸、葡萄糖、总蛋白、钙、钠、钾、氯、无机磷酸盐、镁和酶谱没有不良影响。喂食蘑菇可增加粪便中总脂质和胆固醇的排泄。通过琼脂糖凝胶电泳分离血浆脂蛋白组分，结果表明阿魏侧耳能显著降低血浆β和前β脂蛋白，同时增加α脂蛋白。通过传统苏木精-伊红和油红O染色对肝细胞进行组织学研究，结果显示喂食蘑菇的高胆固醇血症大鼠肝脏组织正常。
本研究表明，5%阿魏侧耳日粮补充剂至少部分通过作用于高胆固醇血症大鼠的致动脉粥样硬化脂质谱而带来健康益处。
