Department of Stomatology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
Chin Med J (Engl). 2011 Dec;124(23):3953-8.
Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration (GTR) and bone grafting. The objective of this study was to investigate the effects of simvastatin/methylcellulose gel on bone regeneration in alveolar defects in miniature pigs.
Alveolar defects were produced in 32 teeth (the third and fourth premolars) of 4 miniature pigs. The 32 experimental teeth were divided into 5 groups comprising control (C) and treatment (T) teeth: (1) empty defects without gel (group C0, n = 4); (2) defects injected with methylcellulose gel (group C1, n = 4); (3) defects injected with 0.5 mg/50 µl simvastatin/methylcellulose gel (group T1, n = 8); (4) defects injected with 1.5 mg/50 µl simvastatin/methylcellulose gel (group T2, n = 8); and (5) defects injected with 2.2 mg/50 µl simvastatin/methylcellulose gel (group T3, n = 8). Every week after surgery, the furcation sites were injected once with gel. At the eighth week after surgery, the 4 pigs were sacrificed and underwent macroscopic observation, descriptive histologic examination, and regenerate bone quantitative histologic examination.
At 8 weeks after surgery, the defect sites in the treatment groups were completely filled in with new bone and fibrous tissue. There was little new bone in the C0 and C1 groups, and only a small number of osteoblasts and proliferative vessels could be seen on microscopic examination.
Miniature pigs are an ideal experimental animal for establishing a model of alveolar defects using a surgical method. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites, because it promotes the proliferation of osteoblasts. The best dose of simvastatin gel to stimulate bone regeneration is 0.5 mg.
目前，用于修复牙槽叉缺损的最常用治疗方法是牙周引导组织再生（GTR）和植骨。本研究旨在探讨辛伐他汀/甲基纤维素凝胶对小型猪牙槽缺损骨再生的影响。
在 4 只小型猪的 32 颗牙齿（第三和第四前磨牙）上制作牙槽缺损。32 颗实验牙分为对照组（C）和治疗组（T）牙齿 5 组：（1）无凝胶的空缺损（C0 组，n=4）；（2）注入甲基纤维素凝胶的缺损（C1 组，n=4）；（3）注入 0.5mg/50μl 辛伐他汀/甲基纤维素凝胶的缺损（T1 组，n=8）；（4）注入 1.5mg/50μl 辛伐他汀/甲基纤维素凝胶的缺损（T2 组，n=8）；（5）注入 2.2mg/50μl 辛伐他汀/甲基纤维素凝胶的缺损（T3 组，n=8）。手术后每周，在分叉部位注入凝胶一次。手术后第 8 周，处死 4 只猪，进行大体观察、描述性组织学检查和再生骨定量组织学检查。
手术后 8 周，治疗组的缺损部位完全被新生骨和纤维组织填充。C0 组和 C1 组新生骨较少，显微镜下仅可见少量成骨细胞和增生血管。
小型猪是通过手术方法建立牙槽缺损模型的理想实验动物。局部应用辛伐他汀/甲基纤维素凝胶可刺激牙槽叉缺损部位的牙槽骨再生，因为它可促进成骨细胞的增殖。刺激骨再生的最佳辛伐他汀凝胶剂量为 0.5mg。
