中国汉族患者中miR-146a和Ets-1基因多态性与富克斯葡萄膜炎综合征无关联。
Lack of association of miR-146a and Ets-1 gene polymorphisms with Fuchs uveitis syndrome in Chinese Han patients.
作者信息
Zhou Qingyun, Kijlstra Aize, Hou Shengping, Yu Hongsong, Zhang Xuedong, Li Xinyu, Xiao Xiang, Yang Peizeng
机构信息
The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, and Chongqing Eye Institute, Chongqing, P.R. China.
出版信息
Mol Vis. 2012;18:426-30. Epub 2012 Feb 11.
PURPOSE
The aim of this study was to investigate the potential association of microRNA-146a (miR-146a) and V-Ets oncogene homolog 1 (Ets-1) gene polymorphisms with Fuchs Uveitis syndrome (FUS).
METHODS
Three single-nucleotide polymorphisms (SNPs), miR-146a/rs2910164, ets-1/rs1128334, and ets-1/rs10893872 were genotyped in 219 Han Chinese patients with FUS and 612 healthy controls using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genotype counts were analyzed by the χ² test.
RESULTS
No significant difference concerning the genotypic and allelic frequencies of rs2910164, rs1128334, and rs10893872 polymorphisms could be found between patients with FUS and the normal controls. Analysis according to gender did not show any influence of sex on the association of miR-146a and Ets-1 with FUS.
CONCLUSIONS
Our results suggest that the investigated three SNPs, miR-146a/rs2910164, ets-1/rs1128334, and ets-1/rs10893872, are not associated with FUS in the Han Chinese population.
目的
本研究旨在探讨微小RNA-146a（miR-146a）和V-Ets癌基因同源物1（Ets-1）基因多态性与富克斯葡萄膜炎综合征（FUS）之间的潜在关联。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对219例汉族FUS患者和612例健康对照者的三个单核苷酸多态性（SNP），即miR-146a/rs2910164、ets-1/rs1128334和ets-1/rs10893872进行基因分型。通过χ²检验分析基因型计数。
结果
在FUS患者和正常对照之间，未发现rs2910164、rs1128334和rs10893872多态性的基因型和等位基因频率有显著差异。按性别分析未显示性别对miR-146a和Ets-1与FUS关联的任何影响。
结论
我们的结果表明，所研究的三个SNP，即miR-146a/rs2910164、ets-1/rs1128334和ets-1/rs10893872，在汉族人群中与FUS无关。
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