Department of Otolaryngology - Head & Neck Surgery, Stanford University School of Medicine, Stanford CA 94305, USA.
Sci Rep. 2011;1:26. doi: 10.1038/srep00026. Epub 2011 Jun 29.
The lack of cochlear regenerative potential is the main cause for the permanence of hearing loss. Albeit quiescent in vivo, dissociated non-sensory cells from the neonatal cochlea proliferate and show ability to generate hair cell-like cells in vitro. Only a few non-sensory cell-derived colonies, however, give rise to hair cell-like cells, suggesting that sensory progenitor cells are a subpopulation of proliferating non-sensory cells. Here we purify from the neonatal mouse cochlea four different non-sensory cell populations by fluorescence-activated cell sorting (FACS). All four populations displayed proliferative potential, but only lesser epithelial ridge and supporting cells robustly gave rise to hair cell marker-positive cells. These results suggest that cochlear supporting cells and cells of the lesser epithelial ridge show robust potential to de-differentiate into prosensory cells that proliferate and undergo differentiation in similar fashion to native prosensory cells of the developing inner ear.
耳蜗再生潜能的缺乏是听力损失永久性的主要原因。尽管在体内处于静止状态,但从新生耳蜗分离出的非感觉细胞在体外增殖,并显示出产生毛细胞样细胞的能力。然而,只有少数非感觉细胞衍生的集落产生毛细胞样细胞,这表明感觉祖细胞是增殖的非感觉细胞的一个亚群。在这里,我们通过荧光激活细胞分选 (FACS) 从小鼠耳蜗中纯化出四种不同的非感觉细胞群体。这四种群体都显示出增殖潜能,但只有较少的上皮嵴和支持细胞能强有力地产生毛细胞标记阳性细胞。这些结果表明,耳蜗支持细胞和较小的上皮嵴细胞显示出强大的潜力,可以去分化为前体细胞,这些前体细胞增殖并以类似于发育中内耳的天然前体细胞的方式进行分化。