Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, 15 Rue de l'Ecole de Médecine, 75006, Paris, France.
Cytotechnology. 1988 Feb;1(2):139-44. doi: 10.1007/BF00146814.
The potentiation or antagonistic effects of Cu, Hg, Pb and Cd salts in the presence of a long-acting anti-rheumatic drug, D-penicillamine (D.P.) were studied on cultured chondrocytes. CuSO(4) (10(-4)M), HgCl(2) (10(-5)M), Pb(CH(3)COO)(2) (10(-3)M) and D.P. (10(-3)M) when used alone caused a small decrease in cell proliferation. The addition of D.P. with Cu, Hg or Pb salts resulted in a marked increase in the extent of growth inhibition. In contrast, CdCl(2) (10(-5)M) produced an important growth inhibitory effect, and D.P. antagonized CdCl(2) action. The CuSO(4) D.P. toxicity was probably due to production of H(2)O(2) in situ. To verify this hypothesis, catalase, responsible for H(2)O(2) metabolism was used, and was found to partially reverse the inhibitory effect of CuSO(4)-D.P.
研究了长效抗风湿药 D-青霉胺（D.P.）存在下，Cu、Hg、Pb 和 Cd 盐对培养软骨细胞的增效或拮抗作用。CuSO4（10-4M）、HgCl2（10-5M）、Pb（CH3COO）2（10-3M）和 D.P.（10-3M）单独使用时，均会导致细胞增殖略有减少。D.P.与 Cu、Hg 或 Pb 盐一起使用会导致生长抑制程度显著增加。相比之下，CdCl2（10-5M）产生了重要的生长抑制作用，而 D.P.拮抗了 CdCl2的作用。CuSO4-D.P.的毒性可能是由于原位生成了 H2O2。为了验证这一假设，使用了负责 H2O2代谢的过氧化氢酶，发现它部分逆转了 CuSO4-D.P.的抑制作用。
