Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
Appl Biochem Biotechnol. 2012 Jul;167(6):1595-602. doi: 10.1007/s12010-012-9572-4. Epub 2012 Feb 26.
The present study deals with the isolation of fungi from soil with the ability to degrade polyurethane (PU). A pure fungal isolate was analyzed for its ability to utilize PU as a sole carbon source in shaking culture for 30 days. Incubation of PU with Aspergillus flavus resulted in 60.6% reduction in weight of PU. The scanning electron microscopy and Fourier transform infrared spectroscopy (FTIR) results showed certain changes on the surface of PU film and formation of some new intermediate products after polymer breakdown. Thermogravimetric curves showed changes between the thermal behavior of the samples that were inoculated with A. flavus and control. FTIR spectra showed detectable changes in control and incubated samples, suggesting that degradation occurs, with the decreased intensity of band at 1,715 cm(-1), corresponding to ester linkages. We have identified an extracellular esterase activity which might be responsible for the polyurethanolytic activity.
本研究从土壤中分离出具有降解聚氨酯（PU）能力的真菌。分析了一株纯真菌分离株在摇瓶培养 30 天内利用 PU 作为唯一碳源的能力。黄曲霉（Aspergillus flavus）与 PU 共培养导致 PU 重量减少了 60.6%。扫描电子显微镜和傅里叶变换红外光谱（FTIR）结果表明，PU 薄膜表面发生了某些变化，并且在聚合物分解后形成了一些新的中间产物。热重曲线表明，接种黄曲霉和对照的样品之间的热行为发生了变化。FTIR 光谱显示对照和孵育样品有可检测的变化，表明发生了降解，与对应酯键的 1715cm(-1) 处的带强度降低有关。我们已经鉴定出一种细胞外酯酶活性，它可能是导致聚氨酯水解的原因。
