Department of Biotechnology, Plant Improvement Division, Tocklai Experimental Station, Jorhat, Assam, India.
Mol Biotechnol. 2013 Mar;53(3):237-48. doi: 10.1007/s12033-012-9517-8.
A cDNA-AFLP approach was used to identify transcript and/or genes specifically expressed in response to drought in tea. Drought was artificially induced and whole genome transcript profiling was done at three different stages-6 days before wilting, 3 days before wilting and at wilting stage of both tolerant and susceptible cultivars, and genetic differences was thus visualized as polymorphisms in the transcriptome. The cDNA-AFLP technique allowed genes and transcripts to be identified in the tolerant genotype (TV-23) whose expression is responsive to drought stress. The cluster analysis revealed two types of clustering-type I separated the tolerant and susceptible cultivar, whereas type II separated the time point of sample and this may be grouped as early and late responsive transcripts. 108 transcript derived fragments were identified as differentially expressed in tolerant genotypes of which 89 sequences could be obtained. Fifty-nine of them showed homology in the public databases. Functional ontology showed genes related to carbohydrate metabolism, response to stress, protein modification process and translation. Cluster I includes five fragments and cluster II includes 25 fragments. Other genes strongly expressed in response to drought in tolerant genotype would help us in identifying and determining the genetic basis of mechanisms involved in conferring drought tolerance in tea.
采用 cDNA-AFLP 方法鉴定了茶树响应干旱胁迫特异性表达的转录本和/或基因。通过人工干旱处理，在萎蔫前 6 天、萎蔫前 3 天和萎蔫期对两个耐受和敏感品种进行全基因组转录谱分析，从而将遗传差异可视化作为转录组中的多态性。cDNA-AFLP 技术允许鉴定出在耐受基因型（TV-23）中表达响应干旱胁迫的基因和转录本。聚类分析显示，两种聚类类型 I 分离了耐受和敏感品种，而 II 类型则分离了样品的时间点，这可能被归类为早期和晚期响应的转录本。在耐受基因型中鉴定出 108 个差异表达的转录本衍生片段，其中 89 个序列可以获得。其中 59 个在公共数据库中具有同源性。功能本体显示与碳水化合物代谢、应激响应、蛋白质修饰过程和翻译相关的基因。簇 I 包括 5 个片段，簇 II 包括 25 个片段。在耐受基因型中强烈表达响应干旱的其他基因将有助于我们鉴定和确定赋予茶树耐旱性的机制的遗传基础。
