Department of Surgery, Sundsvall County Hospital, Sundsvall, Sweden.
Clin Transl Sci. 2012 Feb;5(1):56-9. doi: 10.1111/j.1752-8062.2011.00372.x. Epub 2012 Jan 10.
Abdominal aortic aneurysm (AAA) is a common condition with high mortality when ruptured. Most clinicians agree that small AAAs are best managed by ultrasonographic surveillance. However, it has been stated in recent reviews that a serum/plasma biomarker that predicts AAA rupture risk would be a powerful tool in stratifying patients with small AAA. Identification of such circulating biomarkers has been to date unsuccessful. In this study, we used a proteomic approach to find new, potential plasma AAA biomarker candidates. Prefractionated plasma samples were analyzed by two-dimensional differential in-gel electrophoresis to identify differentially expressed proteins between four patients with small AAA and four controls without aneurysm. Protein spots that differed significantly between patients and controls were selected and identified by mass spectrometry. Three protein spots had significantly different expression between patients and controls. The most interesting finding was that patients with small AAA had increased levels of the enzyme glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) compared with the controls without aneurysm. In conclusion, by using a proteomic approach, this pilot-study provides evidence of GPI-PLD as a novel potential plasma biomarker for AAA.
腹主动脉瘤（AAA）是一种常见疾病，破裂时死亡率很高。大多数临床医生认为，小的 AAA 最好通过超声监测来管理。然而，最近的评论指出，如果有一种能够预测 AAA 破裂风险的血清/血浆生物标志物，那么它将成为对小 AAA 患者进行分层的有力工具。迄今为止，尚未成功鉴定出此类循环生物标志物。在这项研究中，我们使用蛋白质组学方法来寻找新的、潜在的血浆 AAA 生物标志物候选物。通过二维差异凝胶电泳对预分级的血浆样本进行分析，以鉴定出 4 名小 AAA 患者和 4 名无动脉瘤对照之间存在差异表达的蛋白。通过质谱法选择和鉴定差异显著的蛋白点。患者与对照组之间有 3 个蛋白点的表达有显著差异。最有趣的发现是，与无动脉瘤的对照组相比，小 AAA 患者的糖苷磷脂酰肌醇特异性磷脂酶 D（GPI-PLD）酶水平升高。总之，通过蛋白质组学方法，这项初步研究提供了 GPI-PLD 作为 AAA 新型潜在血浆生物标志物的证据。
