Sutor B, ten Bruggencate G
Physiologisches Institut, Universität München, F.R.G.
Neurosci Lett. 1990 Aug 24;116(3):287-92. doi: 10.1016/0304-3940(90)90088-q.
The actions of the reductant ascorbic acid on rat neocortical neurons in vitro was investigated by means of intracellular recordings. At a concentration (500 microM), which reduced the magnitude of dopamine degradation in oxygen-saturated saline solutions by about 50%, ascorbic acid reversibly depressed synaptic potentials and enhanced direct excitability of cortical neurons. The latter effect was not reversible within the observation period. Ascorbic acid did not alter membrane potential and input resistance of the neurons. On the basis of our results we conclude that ascorbic acid is not a useful reductant to avoid oxidation of catecholamines in oxygen-saturated solutions used in electrophysiological experiments in vitro.
通过细胞内记录的方法，研究了还原剂抗坏血酸对体外培养的大鼠新皮质神经元的作用。在浓度为500微摩尔时，抗坏血酸能使氧饱和盐溶液中多巴胺的降解量降低约50%，它可使突触电位可逆性降低，并增强皮质神经元的直接兴奋性。后一种效应在观察期内不可逆。抗坏血酸不改变神经元的膜电位和输入电阻。根据我们的结果，我们得出结论，在体外电生理实验中使用的氧饱和溶液中，抗坏血酸并非避免儿茶酚胺氧化的有效还原剂。
