Yang Dong-Kun, Shin Eun-Kyung, Oh Yoon-I, Lee Kyung-Woo, Lee Chung-San, Kim Seo-Young, Lee Jeong-A, Song Jae-Young
Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea.
J Vet Sci. 2012 Mar;13(1):43-8. doi: 10.4142/jvs.2012.13.1.43.
It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100, and 95%, respectively. The specificities of VI, RT-PCR and RIDA were found to be 100, 100, and 98.9%, respectively. Rabies viruses circulating in Korea were isolated and propagated in murine neuroblastoma (NG108-15) cells with titers ranging from 10(1.5) to 10(4.5) TCID(50)/mL. Although the RIDA findings did not completely coincide with results obtained from FAT, VI, and RT-PCR, RIDA appears to be a fast and reliable assay that can be used to analyze brain samples. In summary, the results from our study showed that VI, RT-PCR, and RIDA can be used as supplementary diagnostic tools for detecting rabies viruses in both laboratory and field settings.
快速准确地诊断狂犬病至关重要。在本研究中，我们评估了四种诊断方法，即间接荧光抗体试验（FAT）、病毒分离（VI）、逆转录聚合酶链反应（RT-PCR）和快速免疫诊断检测（RIDA），以检测动物脑匀浆中的狂犬病病毒。在检测的110份动物脑样本中，根据FAT检测，有20份（18.2%）狂犬病呈阳性。与FAT相比，VI、RT-PCR和RIDA的灵敏度分别为100%、100%和95%。发现VI、RT-PCR和RIDA的特异性分别为100%、100%和98.9%。在韩国流行的狂犬病病毒在小鼠神经母细胞瘤（NG108-15）细胞中分离和增殖，滴度范围为10(1.5)至10(4.5) TCID(50)/mL。虽然RIDA的结果与FAT、VI和RT-PCR获得的结果不完全一致，但RIDA似乎是一种可用于分析脑样本的快速可靠检测方法。总之，我们的研究结果表明，VI、RT-PCR和RIDA可作为在实验室和现场环境中检测狂犬病病毒的补充诊断工具。
