Nuijten P J, Bartels C, Bleumink-Pluym N M, Gaastra W, van der Zeijst B A
Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.
Nucleic Acids Res. 1990 Nov 11;18(21):6211-4. doi: 10.1093/nar/18.21.6211.
The chromosome of Campylobacter jejuni is circular and approximately 1700 kb in circumference. The size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda DNA concatamers and yeast chromosomes to calibrate the size of the fragments. In view of the low (32-35%) G + C content of the campylobacter genome, enzymes that recognizes GC-rich sequences were used. Of the enzymes tested BssHII (G/C(G)CGC), NciI (CC/CGCG) and SalI (G/TCGAC) appeared to be usable. Hybridization of labeled fragments with two or more fragments from digests with a different restriction enzyme gave the information to order the fragments on the C jejuni chromosome. The localization on the genome of the flagellin and ribosomal gene clusters was determined.
