1DIPROVAL, University of Bologna, via Rosselli 107, 42100 Reggio Emilia, Italy.
Animal. 2011 Aug;5(9):1354-60. doi: 10.1017/S1751731111000462.
Lactobacillus rhamnosus GG (LGG) is a probiotic for humans and is normally not found in pigs; however, it has been shown to protect the human-derived intestinal Caco-2 cells against the damage induced by an important intestinal pathogen, enterotoxigenic Escherichia coli F4 (ETEC). An experiment was conducted to test whether the dietary addition of LGG improves the growth and health of weaned pigs when orally challenged by E. coli F4. Thirty-six pigs were weaned at 21 days and assigned to a standard weaning diet with or without 1010 CFU LGG (ATCC 53103) per day. The pigs, individually penned, were orally challenged with 1.5 ml of a 1010 CFU E. coli F4 suspension on day 7 and slaughtered on day 12 or 14. With the addition of LGG, the average daily gain and the average daily feed intake were reduced after the challenge with ETEC and for the entire trial (P < 0.05). The average faecal score tended to worsen from day 11 to the end of the trial and the concentration of ETEC in the faeces tended to increase (P = 0.07) with the LGG supplementation. The counts of lactic acid bacteria, enterobacteria and yeasts in the colonic digesta were not affected. The pH values in ileal, colonic and caecal digesta, and the small intestine size were also unchanged. Regardless of the site of measurement (duodenum, jejunum or ileum), a trend of decreased villus height was seen with LGG (P = 0.10). Crypt depth and villus to crypt ratio were unchanged by the diet. A gradual increase of total seric IgA was seen after 1 week and after the challenge, in the control (P < 0.05), but not in the treated group. After the challenge, the LGG reduced the total IgA in the blood serum (P < 0.05), v. the control. The total IgA in the saliva and in the jejunum secretion were not affected by the diet. The F4-specific IgA activity was not affected by the diet at all the samplings. Our result shows that, the administration of LGG do not prevent or reduce the detrimental effect of the E. coli F4 infection on the growth performance and health status of weaned piglet.
鼠李糖乳杆菌 GG（LGG）是一种人类益生菌，通常不存在于猪中；然而，它已被证明可保护源自人类的肠 Caco-2 细胞免受重要肠病原体产肠毒素性大肠杆菌 F4（ETEC）的损伤。进行了一项实验，以测试当仔猪经口挑战 ETEC 时，日粮中添加 LGG 是否可以改善其生长和健康。36 头仔猪于 21 日龄断奶，并分配到标准断奶日粮中，或每日添加 1010 CFU LGG（ATCC 53103）。单独饲养的仔猪于第 7 天口服 1.5ml 1010 CFU ETEC F4 悬浮液，并于第 12 或 14 天屠宰。添加 LGG 后，在 ETEC 挑战后和整个试验期间，平均日增重和平均日采食量均降低（P<0.05）。粪便评分从第 11 天开始趋于恶化，粪便中 ETEC 的浓度也趋于增加（P=0.07）。日粮添加 LGG 并未影响结肠内容物中的乳酸菌、肠杆菌和酵母菌计数。回肠、结肠和盲肠内容物的 pH 值以及小肠大小也没有变化。无论测量部位（十二指肠、空肠或回肠）如何，添加 LGG 都会导致绒毛高度呈下降趋势（P=0.10）。日粮未改变隐窝深度和绒毛-隐窝比。在对照组中，1 周后和挑战后，总血清 IgA 逐渐增加（P<0.05），但在处理组中没有增加。在挑战后，与对照组相比，LGG 降低了血清中的总 IgA（P<0.05）。饮食未影响唾液和空肠分泌物中的总 IgA。在所有采样中，F4 特异性 IgA 活性均不受饮食影响。我们的结果表明，LGG 的给药并不能预防或减轻 ETEC F4 感染对断奶仔猪生长性能和健康状况的不利影响。
