Thibodeau Alexandre, Fravalo Philippe, Laurent-Lewandowski Sylvette, Guévremont Evelyne, Quessy Sylvain, Letellier Ann
Université de Montréal, département de microbiologie et de pathologie, St-Hyacinthe, Québec.
Can J Vet Res. 2011 Oct;75(4):298-307.
The objective of this study was to estimate the presence of the important foodborne pathogen Campylobacter jejuni in organically raised chickens in the province of Quebec. The recovered isolates were further characterized for their antimicrobial resistance profile, autoagglutination property and chemotaxis. Antimicrobial resistance was evaluated using agar dilution for: tetracycline, erythromycin, chloramphenicol, ciprofloxacin, gentamicin, nalidixic acid, clindamycin, ampicillin, azithromycin, bacitracin, and ceftiofur. Autoagglutination was measured by monitoring optical density changes in a bacterial suspension after 3 h of incubation at room temperature. Chemotaxis was evaluated after a contact time of 3 h between isolates and mucin, using a quantitative protocol. A total of 10 lots of chickens was sampled in August and September 2009; half of them were positive for the presence of C. jejuni. Antimicrobial resistance was found only for tetracycline (44%), erythromycin (6%), azithromycin (6%) and clindamycin (2%). Variation was observed in the minimum inhibitory concentrations (MICs) for ceftiofur and bacitracin, for which C. jejuni possess intrinsic resistance. Autoagglutination and chemotaxis varied among isolates and lot-level differences in these were observed. Autoagglutination and chemotaxis levels appeared as independent isolate properties. Further monitoring and characterization of isolates originating from organic chickens is of interest since this type of production might represent another source of exposure of consumers to a variety of the foodborne pathogen C. jejuni.
本研究的目的是评估魁北克省有机饲养鸡中重要的食源性病原体空肠弯曲菌的存在情况。对分离出的菌株进一步进行抗菌药物耐药性、自凝特性和趋化性分析。采用琼脂稀释法评估对以下抗菌药物的耐药性：四环素、红霉素、氯霉素、环丙沙星、庆大霉素、萘啶酸、克林霉素、氨苄西林、阿奇霉素、杆菌肽和头孢噻呋。通过监测室温孵育3小时后细菌悬液的光密度变化来测定自凝性。采用定量方案，在分离株与粘蛋白接触3小时后评估趋化性。2009年8月和9月共采集了10批鸡的样本；其中一半空肠弯曲菌检测呈阳性。仅发现对四环素（44%）、红霉素（6%）、阿奇霉素（6%）和克林霉素（2%）有耐药性。观察到头孢噻呋和杆菌肽的最低抑菌浓度（MIC）存在差异，空肠弯曲菌对这两种药物具有固有耐药性。分离株之间自凝性和趋化性存在差异，且批次间亦有差异。自凝性和趋化性水平似乎是分离株的独立特性。鉴于这种生产类型可能是消费者接触多种食源性病原体空肠弯曲菌的另一个来源，因此对源自有机鸡的分离株进行进一步监测和特性分析很有必要。
