Thornton Emily E, Krummel Matthew F, Looney Mark R
Department of Pathology, University of California, San Francisco, California.
Department of Medicine, University of California, San Francisco, California.
Curr Protoc Cytom. 2012 Apr;Chapter 12:12.28.1-12.28.12. doi: 10.1002/0471142956.cy1228s60.
Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung.
活体成像对于确定组织环境中细胞的动态变化和空间相互作用至关重要。在肺部,由于通气和心脏收缩引起的运动,这已被证明是困难的。在本章中,我们报告了对离体活肺切片和完整小鼠肺进行成像的方案。