Chu Kaiqin, Smith Zachary J, Wachsmann-Hogiu Sebastian, Lane Stephen
Center for Biophotonics Science and Technology, University of California, Davis, California 95817, USA.
J Opt Soc Am A Opt Image Sci Vis. 2012 Mar 1;29(3):344-51. doi: 10.1364/JOSAA.29.000344.
We report a scheme to achieve resolution beyond the diffraction limit in spatial light interference microscopy (SLIM). By adding a grating to the optical path, the structured illumination technique can be used to improve the resolution by a factor of 2. We show that a direct application of the structured illumination technique, however, has proved to be unsuccessful. Through two crucial modifications, namely, one to the pupil plane of the objective and the other to the demodulation procedure, faithful phase information of the object is recovered and the resolution is improved by a factor of 2.
我们报告了一种在空间光干涉显微镜(SLIM)中实现超越衍射极限分辨率的方案。通过在光路中添加一个光栅,结构照明技术可用于将分辨率提高两倍。然而,我们表明直接应用结构照明技术已被证明是不成功的。通过两个关键修改,即一个针对物镜的光瞳平面,另一个针对解调过程,物体的真实相位信息得以恢复,并且分辨率提高了两倍。