Department of Periodontics, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
Braz Oral Res. 2012 Mar-Apr;26(2):158-64. doi: 10.1590/s1806-83242012000200012.
The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18), and Test Group - OE 1493-treated (n = 18). The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.
本研究旨在评估从Ipomoea alba L.（旋花科或 OE 1493）获得的有机提取物对实验性牙周病大鼠的影响。在 36 只 Wistar 大鼠中诱导牙周炎：随机选择第一下颌磨牙结扎，而对侧磨牙不结扎。动物随机分为两组，每天局部治疗 3 次，共 11 天，如下所示：对照组-载体处理（n = 18）和试验组-OE 1493 处理（n = 18）。第 12 天处死大鼠。使用标准化照片从牙骨质-釉质交界处到牙槽嵴进行形态计量测量，以确定牙槽骨丧失。在 OE 1493 处理后进行单次和多次急性毒性测定。形态计量分析表明，与对照组相比，局部给予 OE 1493 对减少骨丢失没有影响（p > 0.05）。此外，OE 1493 没有毒性。在本研究的范围内，可以得出结论，OE 1493 按照本研究中使用的方案给药时，对结扎诱导的大鼠牙周炎的进展没有任何积极影响。
