de Castilho Adriana Lígia, da Silva Juliana Paola Correa, Saraceni Cintia Helena Coury, Díaz Ingrit Elida Collantes, Paciencia Mateus Luís Barradas, Varella Antonio Drauzio, Suffredini Ivana Barbosa
Programa de Graduação em Odontologia Universidade Paulista São PauloSP Brazil Programa de Graduação em Odontologia, Universidade Paulista, São Paulo, SP, Brazil.
Laboratório de Extração Núcleo de Pesquisas em Biodiversidade São PauloSP Brazil Laboratório de Extração, Núcleo de Pesquisas em Biodiversidade, São Paulo, SP, Brazil.
Braz J Microbiol. 2014 Oct 9;45(3):769-79. doi: 10.1590/s1517-83822014000300002. eCollection 2014.
Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC(®) 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by (13)C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.
先前对2200种植物提取物的研究表明，从巴西森林植物中提取的25种提取物具有抗肠球菌活性。在本研究中，使用浮游粪肠球菌ATCC(®) 29212™对这些提取物进行了微量稀释肉汤试验（MDBA）和纸片扩散试验（DDA），并在薄层色谱法（TLC）和高效液相色谱法（HPLC）中进行了植物化学分析。从白花曼陀罗（最低抑菌浓度<40μg/mL）、杯萼双瓣楠（最低抑菌浓度≤40μg/mL）和绯红莫伦藤（40<最低抑菌浓度<80μg/mL；最低杀菌浓度=80μg/mL）中获得的三种提取物在MDBA中显示出显著的杀菌活性，从白花曼陀罗（直径14.04±0.55mm）、球花豆（直径14.43±0.33mm和12.18±0.28mm）和红康纳树（直径13.13±0.18mm）中获得的四种提取物在DDA中具有活性。与各自的粗提物相比，从致密番石榴（平均直径16.78mm）和美丽密花豆（平均直径15.97mm）中获得的水提取物在分馏后显示出增强的抗菌活性。在活性提取物的一些残渣中观察到了抗氧化活性。TLC分析表明，活性提取物中可能存在酚类化合物。从球花豆中分离出三种分子，并通过(13)C核磁共振鉴定为羽扇豆醇、α-香树脂醇和3β-羟基谷甾-5-烯。目前的化学和生物学研究结果表明，这些提取物是牙髓治疗中引入新型抗肠球菌化合物的潜在来源。
