罗氏 LightCycler 实时 PCR 检测在诊断肺外结核中的性能。
Performance of the Roche LightCycler real-time PCR assay for diagnosing extrapulmonary tuberculosis.
机构信息
Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
出版信息
J Clin Microbiol. 2012 Jun;50(6):2100-3. doi: 10.1128/JCM.00252-12. Epub 2012 Apr 11.
Abstract
The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.
摘要
罗氏 LightCycler 分枝杆菌检测分子检测法用于检测结核分枝杆菌、鸟分枝杆菌和堪萨斯分枝杆菌，适用于组织标本。该方法在检测淋巴结和脑脊液标本时性能良好，而在检测肺、肝和骨髓核心活检标本时性能稍差，但如果结合临床对结核病的怀疑，它可以辅助患者管理。
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