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罗氏 LightCycler 实时 PCR 检测在诊断肺外结核中的性能。

Performance of the Roche LightCycler real-time PCR assay for diagnosing extrapulmonary tuberculosis.

机构信息

Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

出版信息

J Clin Microbiol. 2012 Jun;50(6):2100-3. doi: 10.1128/JCM.00252-12. Epub 2012 Apr 11.

DOI:10.1128/JCM.00252-12
PMID:22495565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3372101/
Abstract

The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.

摘要

罗氏 LightCycler 分枝杆菌检测分子检测法用于检测结核分枝杆菌、鸟分枝杆菌和堪萨斯分枝杆菌，适用于组织标本。该方法在检测淋巴结和脑脊液标本时性能良好，而在检测肺、肝和骨髓核心活检标本时性能稍差，但如果结合临床对结核病的怀疑，它可以辅助患者管理。

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Comparison of Xpert MTB/RIF with other nucleic acid technologies for diagnosing pulmonary tuberculosis in a high HIV prevalence setting: a prospective study.在高 HIV 流行地区比较 Xpert MTB/RIF 与其他核酸技术诊断肺结核的前瞻性研究。
PLoS Med. 2011 Jul;8(7):e1001061. doi: 10.1371/journal.pmed.1001061. Epub 2011 Jul 26.
3
Xpert MTB/RIF®, a novel automated polymerase chain reaction-based tool for the diagnosis of tuberculosis.Xpert MTB/RIF®，一种新型自动化聚合酶链反应技术，用于结核病的诊断。
Int J Tuberc Lung Dis. 2011 Jul;15(7):988-9. doi: 10.5588/ijtld.10.0574.
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